中国六省致病小肠结肠炎耶尔森菌的脉冲场凝胶电泳分析

目的 对中国致病小肠结肠炎耶尔森菌分离株进行脉冲场凝胶电泳分析。方法 参照美国疾病预防控制中心PulseNet实验方法对中国6省165株致病小肠结肠炎耶尔森菌进行脉冲场凝胶电泳分析，使用BioNumerics软件进行聚类分析，并按照PulseNet命名原则对带型进行命名。结果 将114株O：3血清型小肠结肠炎耶尔森菌分成25个带型；K6GNllC30012（50株）、K6GNllC30015（19株）及K6GNllC30016（10株）是其主要带型，三个主要带型之间聚类相似性很高。将51株O：9血清型小肠结肠炎耶尔森菌分为14个带型；其中K6GNllC90004（22株）与K6GNllC90010（13株）是其主要带型，K6GNllC90004与K6GNllC90010带型之间有一定的差异。O：3与O：9血清型的主要带型之间有明显的差异。结论 O：3血清型的小肠结肠炎耶尔森菌可能起源于一个克隆系，且在不同地区和年代变异很小；O：9血清型的小肠结肠炎耶尔森菌可能起源于两个不同的克隆系，且各自有一定的变异。O：3与O：9血清型的小肠结肠炎耶尔森菌亲缘关系较远。

Molecular typing of the pathogenic Yersinia enterocolitica strains with pulsed field gel electrophoresis isolated in China

Objective To investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China, using pulsed field gel electrophoresis(PFGE) and standardized PFGE method as well as typing database of Yersinia enterocolitica. Methods PFGE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet ,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0, Applied Maths BVBA, Belium). Results 114 O:3 Yersinia enterocolitica strains were typed by 25 patterns to have found that K6GN11C30012(50 strains), K6GN11C30015( 19 strains) and K6GN11C30016(10 strains) were the major patterns. K6GN11C30012 had 92.2% cluster similarity with K6GN11C30009-K6GN11C30023. This clone included 91.23% strains of 114 O:3 Yersinia enterocolitica strains. 51 O:9 Yersinia enterocolitica strains were typed by 14 patterns; K6GN11C90004 (22 strains) and K6GN11C90010 (13 strains)were the major patterns. K6GN11C90004 had 81.8% cluster similarity with K6GN11C90010 patterns. The major patterns of O:3 and O-9 serotypes were quite different. Conclusion O:3 Yersinia enterocolitica strains might originate from the same clone and had very few variation in different years and provinces but O-9 Yersinia enterocolitica strains from two different clones with some changes.