LC/MS联用法测定人血浆中右美沙芬的浓度及其药动学研究

目的:建立以液/质联用法测定人血浆中右美沙芬浓度的方法,并研究其在健康人体的药动学。方法:以曲马多为内标,血浆样品经液-液萃取后,采用Zorbax Extend-C18色谱柱进行分离,通过Q TRAPTM四极杆-线性离子阱质谱仪,以多反应检测方式进行测定。结果:右美沙芬的线性范围为0.05~10.0ng.mL-1(r=0.999 5);平均相对回收率在97.7%~99.5%之间;日内、日间精密度的RSD均小于6.8%,右美沙芬的定量下限为0.05ng.mL-1。结论:本法快速、简便、准确、灵敏,适用于右美沙芬的人体药动学研究。

Determination of Dextromethorphan in Human Plasma by LC/MS and Study on Its Pharmacokinetics

OBJECTIVE: To establish a LC/MS method for the determination of dextromethorphan in human plasma and to study its pharmacokinetics in healthy Chinese volunteers.METHODS: Taking tramadol as internal standard,after liquidliquid extraction,the plasma sample was separated on Zorbax Extend-C18 column.Detection was carried out by multiple reaction monitoring(MRM) on a Q TRAPTM LC/MS system.RESULTS: Standard curve was linear in the range of 0.05～ 10.0ng· mL-1(r=0.999 5).The average relative recovery was 97.7% ～ 99.5%.RSD of inter-day and intra-day were all less than 6.8%.The lower limit of quantification was 0.05ng· mL-1.CONCLUSION: The method is rapid,simple,accurate and sensitive,and suitable for pharmacokinetic studies of dextromethorphan.