| Protective Effect of Paeoniflorin against Optic Nerve Crush |
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| 引用本文： | 李敬,熊新春,刘云海. Protective Effect of Paeoniflorin against Optic Nerve Crush[J]. 华中科技大学学报(医学英德文版), 2007, 27(6): 650-652. DOI: 10.1007/s11596-007-0607-y |
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| 作者姓名： | 李敬 熊新春 刘云海 |
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| 作者单位： | Department of Pharmacy Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Ophthalmology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Pharmacy Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430022 China，Wuhan 430030 China |
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| 摘 要： | In order to evaluate the efficacy of traditional paeonia extract paeoniflorin against optic nerve crush, 16 Brown Norway rats were divided into two groups at random, with 8 rats in each group. In paeoniaflorin-treated group, 2 mg paeoniaflorin （total volum： 1 mL） was injected into rat＇s peritoneum one time a day for a period of 8 days. Rats in untreated group were given a single dose of vehicle. The optic nerve was crushed by a special forceps for 30 s in the left eye and a sham procedure was performed in the right eye on the 2nd day after the first injection. The retrograde fluorogold labeling of ganglion cells was conducted 5 days after optic nerve crush. The whole retina was flat-mounted thereafter. The ganglion cells that survived the crush were counted under fluorescent microscope by using an automatic counting software. As compared with the contralateral eye, the survival rate of ganglion cells in the left eye increased from 40.22% to 64.53% with a significant difference found between them （t=2.55, P=0.023）. The results suggested that the paeonia extract paeoniflorin possessed a protective effect against optic nerve crush.
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| 关 键 词： | 视神经变形 视网膜神经中枢细胞 神经退化 眼科学 |
| 收稿时间： | 2007-10-31 |
Protective effect of paeoniflorin against optic nerve crush |
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| Li Jing,Xiong Xinchun,Liu Yunhai. Protective effect of paeoniflorin against optic nerve crush[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2007, 27(6): 650-652. DOI: 10.1007/s11596-007-0607-y |
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| Authors: | Li Jing Xiong Xinchun Liu Yunhai |
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| Affiliation: | 1. Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | In order to evaluate the efficacy of traditional paeonia extract paeoniflorin against optic nerve crush, 16 Brown Norway rats were divided into two groups at random, with 8 rats in each group. In paeoniaflorin-treated group, 2 mg paeoniaflorin (total volum 1 mL) was injected into rat's peritoneum one time a day for a period of 8 days. Rats in untreated group were given a single dose of vehicle. The optic nerve was crushed by a special forceps for 30 s in the left eye and a sham proce-dure was performed in the right eye on the 2nd day after the first injection. The retrograde fluorogold labeling of ganglion cells was conducted 5 days after optic nerve crush. The whole retina was flat-mounted thereafter. The ganglion cells that survived the crush were counted under fluorescent microscope by using an automatic counting software. As compared with the contralateral eye, the survival rate of ganglion cells in the left eye increased from 40.22% to 64.53% with a significant dif- ference found between them (t=2.55, P=0.023). The results suggested that the paeonia extract paeoniflorin possessed a protective effect against optic nerve crush. |
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| Keywords: | paeoniflorin optic nerve crush retina ganglion cell retrograde labeling |
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