Determination of growth fraction and cell density to evaluate the potential growth of human oligodendroglial and astrocytic tumours |
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Authors: | Laurence Gordower Christine Decaestecker Maria-Beatriz Lopes Isabelle Camby Nathalie Nagy Christine François Patrick Cras Jean-Jacques Martin Jacques Brotchi Robert Kiss Isabelle Salmon |
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Institution: | (1) Laboratory of Histology, Faculty of Medicine, Free University of Brussels (ULB), 808 route de Lennik, B-1070 Brussels, Belgium e-mail: rkiss@med.ulb.ac.be; Tel.:+322 555 63 80 Fax: 322 555 62 85, BE;(2) Division of Neuropathology, Department of Pathology, University of Virginia Health Sciences Center, Charlottesville, Virginia USA, US;(3) Department of Pathology, Erasmus University Hospital, Free University of Brussels (ULB), Brussels, Belgium, BE;(4) Department of Neuropathology, University of Antwerp (UIA), Antwerp, Belgium, BE;(5) Department of Neurosurgery, Erasmus University Hospital, Free University of Brussels (ULB), Brussels, Belgium, BE |
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Abstract: | The object of this work was Purpose: to develop a methodology that enables net tumour growth, a balance between actual tumour growth and tumour cell loss, to
be approximately evaluated. Methods: The methodology proposed relies on detecting the growth fraction immunohistochemically by means of MIB-1 antibody labelling
combined with cell density determination, carried out on 5-μm-thick Feulgen-stained histological sections with computer-assisted
microscopy. The series investigated included 25 oligodendrogliomas (OLG-II), 9 anaplastic oligodendrogliomas (OLG-III), 13
astrocytomas (AST), 14 anaplastic astrocytomas (ANA) and 8 mixed oligoastrocytomas (OLG-AST). Results: The results show that the biological characteristics of some cases were in total accordance with their histopathological
diagnoses. This was the case for the “weakly proliferating weakly dense” OLG-II and AST-II tumours, and for the “highly proliferating
highly dense” OLG-III and AST-III ones. In contrast, the biological characteristics of some cases seemed to contradict the
histopathological case labels. This was the case for the “highly proliferating highly dense” OLG-II and AST-II tumours, the
biological aggressiveness of which would be undervalued on the basis of the morphology-based grading system alone, and also
for the “weakly proliferating weakly dense” OLG-III and AST-III tumours, the aggressiveness of which would be overvalued.
Conclusions: Combining the determinations of the MIB-1 and the cell density variables appears to be satisfactory in terms of the cell
kinetic characterization of glial tumours as a complement to the prognostic information given by a morphology-based grading
system alone.
Received: 6 January 1998 / Accepted: 3 April 1998 |
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Keywords: | Cell loss Growth fraction Cell proliferation Cell density Gliomas |
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