PCR法和14C呼气试验检测幽门螺杆菌的比较

用聚合酶链反应（ＰＣＲ）和１４Ｃ呼气试验（１４Ｃ－ＵＢＴ）对６６例上消化道疾病患者进行幽门螺杆菌（Ｈｐ）检测，结果ＰＣＲ检出率为６９．７％，１４Ｃ－ＵＢＴ检出率为７１．２％，ＰＣＲ、１４Ｃ－ＵＢＴ两种方法诊断Ｈｐ感染的敏感性均为１００％，特异性分别为９５．２％和９０．５％，精确性分别为９８．５％和９７．０％；慢性萎缩性胃炎和慢性浅表性胃炎Ｈｐ检出率分别为７７．８％和６５．０％，两者无显著性差异；Ｈｐ感染无性别差异。研究结果表明，１４Ｃ－ＵＢＴ和采用自制的采样器获取胃粘膜组织进行ＰＣＲ检测Ｈｐ均具有非创伤性、简便、高敏感性和特异性的优点，后者还可进行Ｈｐ细胞毒株和非细胞毒株的区分，克服了１４Ｃ－ＵＢＴ具有一定的放射性和使用有毒试剂的缺点

Comparison of PCR with 14 C urea breath test for the detection of Helicobacter pylori

In order to compare the value of polymerase chain reaction(PCR)with 14 C urea breath test( 14 C UBT)for the diagnosis of Helicobacter pylori (Hp),specimens from 66 patients with upper gastrointestinal diseases were detected by both methods.The results showed the sensitivity of the PCR and 14 C UBT for the diagnosis of Hp infection were all 100%,the specificity were 95 2% and 90 5%,and the accuracy were 98 5% and 97 0%,respectively.There were not statistically significant difference between PCR and 14 C UBT,and also between males and females.This study shows that 14 C UBT and PCR assay with selfmade sampling tool for collecting gastric secretion have the advantages of being noninvasive,simple,high sensitivity and specificity.The latter can be used to discriminate cytotoxic strains with no cytotoxic strains of Hp,and that conquer the shortages of 14 C UBT being radioactive and using poisonous reagents.PCR assay is recommendable to apply for the diagnosis of Hp infection.