Effects of RU486 in the expression of progesterone receptor isoforms in the hypothalamus and the preoptic area of the rat during postpartum estrus |
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Authors: | Carrillo-Martínez Graciela Elena Gómora-Arrati Porfirio González-Arenas Aliesha Roldán-Roldán Gabriel González-Flores Oscar Camacho-Arroyo Ignacio |
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Institution: | a Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, México DF 04510, Mexico b Centro de Investigación en Reproducción Animal, CINVESTAV - Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico c Laboratorio de Neurobiología de la Conducta, Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico |
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Abstract: | In several mammalian species females undergo postpartum estrus, a brief period of ovulation and sexual receptivity that in rats usually occurs during the first 24 h following parturition. The maximal lordotic expression occurs at 12 h after the initiation of parturition and depends on intracellular progesterone receptor (PR). We studied the regulation of PR expression by its antagonist, RU486 in the hypothalamus and the preoptic area of the rat during postpartum estrus by Western blot. Adult female rats were treated with RU486 (1.25 and 5 mg) 3 h after parturition, and Western blot was performed to assess the expression of PR-A and PR-B at 12 h postpartum. RU486 (1.25 and 5 mg) reduced the expression of PR-A (63% and 95%) and that of PR-B (75% and 99%), respectively in the preoptic area whereas it had no effects in the hypothalamus. These results suggest a differential regulation of PR expression in the rat brain during postpartum estrus. |
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Keywords: | Hypothalamus Lordosis Postpartum estrus Preoptic area Progesterone Progesterone receptor RU486 |
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