An autoradiographic study of tryptophane-H3 incorporation into rat enamel and dentine matrices |
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| Authors: | W R Cotton S M Hefferren |
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| Affiliation: | 1. Dental Research Department, Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland 20014, USA;2. American Dental Association, Chicago, Illinois 60611, U.S.A.;2. Servicio de Apoyo a la Investigación Tecnológica, Universidad Politécnica de Cartagena, Edificio I+D+i, Calle Linterna s/n, Campus Muralla de Mar 30202 Cartagena, Murcia, Spain;3. Servei de Recursos Científics i Técnics, Universitat Rovira i Virgili, Avda, Països Catalans 26, 43007 Tarragona, Spain;1. Faculty of Dentistry, Universidad Cooperativa de Colombia. Carrera 47 # 37 sur 18, Envigado, Colombia;2. Production Engineering Department, Universidad Eafit, Carrera 49 # 7 sur 50, Medellín, Colombia |
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| Abstract: | l-tryptophane-H3 was administered intraperitoneally, 1 μc/g of body weight, to 9-day-old NMRI-D rats. The rats were killed, 1, 4, 8 and 24 hr and 7 days post-injection. The incorporation and distribution of tryptophane-H3 were evaluated in autoradiographs of the developing mandibular first molars. At 1 hr the cytoplasm labelling of the ameloblasts was considerably greater than the odontoblast labelling. The cytoplasm grain density over the ameloblasts gradually decreased as the labelling of the enamel matrix increased. At 4 and 8 hr the labelling was primarily concentrated over the predentine and the first 4 layers (layer = 9·3μ × 130.2μ) of enamel matrix. At 7 days the dense labelling of the enamel matrix was diffusely spread throughout; whereas, the dentine remained sparsely labelled. The observation that tryptophane is a component of the organic matrices of enamel in developing rat molars is confirmed by autoradiography. |
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