食管鳞癌p27的表达及其临床意义

目的　p2 7可抑制细胞周期的向前推进 ,作用于细胞周期的分子更能较精确地反映肿瘤的生物学行为。即可为临床提供更为有价值的信息。本研究的目的是观察p2 7在食管癌的表达特性及阐明该分子标志物与临床病理因素之间的关系。方法　 89例 (5 6例男性、33例女性 )食管鳞癌行外科手术切除。其标本用p2 7抗体行免疫组织化学染色。p2 7表达程度以标记指数表示。结果　p2 7免疫染色在瘤与非瘤组织均固定于细胞核。p2 7的阳性免疫染色在高分化鳞癌 (4 7 3% ,14 /32 )多于其它类型 (35 5 % ,11/31;2 3 1% ,6 /2 6 ) ,有统计学意义 (P <0 0 5 )。结论　食管鳞癌细胞的增殖状况信息可由p2 7的表达程度提示 ,p2 7表达缺失提示食管鳞癌分化差

Expression of p27 in squamous cell carcinoma of the esophagus

Objective P27 is associatfed with cell proliferation.Tumor cell cycle analysis has indicated that tumors with a higher proliferation rate showed more aggresisve clinical behavior.Therefore,it is important to understand cell proliferation of this disease in clinical practice.The objective of this study was to observe the character of expression of P27 in esophageal carcinoma and to clarify the relation between clinicopathologic and this factor.Methods Immunohistochemistry for P27 was carried out.P27 expression was examined and detailed pathologic examinations were conducted on tumors from the patients (56 males and 33 females with surgically resected esophageal squamous cell carcioma).Results P27 immunostaining were all comfined to the nuclei in both neoplastic and non-neoplastic cells.The P27 positive immunostaining in the nuclei was significantly higher in well differentiated sequamous cell carcinoma (SCC43.7%,14/32) than in the other tumer types (35.5%,11/31;23.1%,6/26,P<0.05).Conclusion The expression levels of P27 may imply the proliferative activity of caner cells in patient with SCC of esophagus.The loss of P27 expression correlates with poor SCC differentiation in patients with esophageal carcinoma.