t10,c12-共轭亚油酸对骨骼肌细胞脂肪酸代谢的影响

目的通过研究t10,c12-共轭亚油酸(t10,c12-CLA)对高游离脂肪酸环境中骨骼肌细胞脂肪酸代谢的影响,探讨t10,c12-CLA改善脂代谢的作用机制。方法体外培养成熟的C2C12骨骼肌细胞,通过施加软脂酸在体外模拟高游离脂肪酸环境,再给予不同浓度的t10,c12-CLA(20、30和40μmol/L),通过荧光免疫法利用激光共聚焦显微镜观察t10,c12-CLA对细胞脂肪酸转运蛋白(FAT/CD36)的影响,应用Western blot法检测t10,c12-CLA对C2C12细胞脂肪酸代谢相关酶及AMP激活的蛋白激酶(AMPK)的作用。结果t10,c12-CLA剂量依赖性的增加了高游离脂肪酸环境中细胞对脂肪酸的摄取,抑制了乙酰辅酶A羧化酶(ACC)的活性,增加了肉碱酰基转移酶-1(CPT-1)的表达,并且激活了AMPK。结论t10,c12-CLA可通过激活AMPK增强细胞对脂肪酸的氧化代谢。

Effect of t10,c 12-conjugated linoleic acid on fatty acid metabolism in C2 C12 myotubes

Objective To study the effect of t10,c12-conjugated linoleic acid (t10,c12-CLA) on fatty acid metabolism in myotubes under the high free fatty acid condition. Methods We used palmitate to simulate a high free fatty acid condition for C_2C_ 12 myotubes in vitro and administered t10,c12-CLA across the groups. The expression and translocation of FAT/CD_ 36 were determined by fluorimetric immunifaction using laser scanning confocal microscope. A series of enzymes related to fatty acid metabolism, together with AMP-activated protein kinase (AMPK), were determined by western blot. Results t10,c12-CLA dose-dependently increased the translocation of FAT/CD_ 36 to cellular membranes, and increased the expression of p-ACC and CPT-1 in myotubes under the high free fatty acid condition. Moreover, t10,c12-CLA activated the AMPK in myotubes. Conclusion CLA can increased the fatty acid oxidation in myotubes via activating AMPK