Cytokine-induced apoptotic cell death in a mouse pancreatic beta-cell line: inhibition by Bcl-2 |
| |
Authors: | Dr. H. Iwahashi T. Hanafusa Y. Eguchi H. Nakajima J. Miyagawa N. Itoh K. Tomita M. Namba M. Kuwajima T. Noguchi Y. Tsujimoto Y. Matsuzawa |
| |
Affiliation: | (1) Second Department of Internal Medicine, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565 Osaka, Japan;(2) Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan;(3) Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Osaka, Japan;(4) Department of Laboratory Medicine, School of Medicine, University of Tokushima, Tokushima, Japan;(5) Department of Biochemistry, Fukui Medical School, Fukui, Japan |
| |
Abstract: | Summary Cytokines are thought to contribute to the induction of pancreatic beta-cell destruction in insulin-dependent diabetes mellitus. The molecular mechanisms that underlie beta-cell death were investigated by studying cytokine-induced cell death in beta-cell lines. A combination of three cytokines (interleukin-1, tumour necrosis factor-, and interferon-) induced apoptotic cell death in the mouse pancreatic beta-cell line TC1, as judged from the appearance of cells with hypodiploid nuclei and oligonucleosomal DNA fragmentation. The same treatment also induced apoptosis in the mouse pancreatic alpha-cell line TC1 and the NOD/Lt mouse beta-cell line NIT-1, although to a lesser extent than in TC1 cells. The abundance of endogenous Bcl-2 in TC1 cells was lower than that in the other two cell lines. Overexpression of human Bcl-2 in TC1 cells partially protected them from cytokine-induced cell death. These results suggest that apoptosis may be responsible, at least in part, for cytokine-induced beta-cell destruction and that Bcl-2 prevents apoptosis in pancreatic islet cells.Abbreviations IDDM Insulin-dependent diabetes mellitus - IL interleukin - TNF tumour necrosis factor - IFN interferon - FBS fetal bovine serum - ATA aurintricarboxylic acid - CHX cycloheximide - PI propidium iodide |
| |
Keywords: | Pancreatic beta cell Bcl-2 apoptosis cytokine interleukin-1 tumour necrosis factor interferon- /content/u7551p02k616871p/xxlarge947.gif" alt=" gamma" align=" MIDDLE" BORDER=" 0" > |
本文献已被 SpringerLink 等数据库收录! |
|