乙酸铅对脑脉络丛Z310细胞毒性作用

目的初步探讨乙酸铅诱导大鼠脑脉络丛Z310细胞的低剂量兴奋作用和高剂量抑制作用。方法以浓度为0、0.000 2、0.002、0.02、0.2、2、20、200、500μmol/L的乙酸铅分别染毒Z310细胞12和24h,用噻唑蓝(MTT)法和2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8)法检测细胞生存情况,并观察各剂量组细胞形态变化。结果 0.02μmol/L乙酸铅染毒24 h,MTT法检测Z310细胞存活率为107.06%,0.2μmol/L染毒组24 h时细胞存活率升高为110.91%;>2μmol/L乙酸铅染毒时,细胞存活率随剂量增加而降低;染毒24 h时,200、500μmol/L乙酸铅组细胞存活率(MTT法)分别下降至79.37%和76.81%(P<0.01);染毒12、24 h时,200、500μmol/L乙酸铅组细胞存活率(WST-8法)分别下降至81.67%和72.36%及56.89%和44.05%(P<0.05)。结论低剂量乙酸铅可引起Z310细胞兴奋效应;高剂量乙酸铅引起Z310细胞抑制效应;WST-8法检测细胞存活率较MTT法更敏感。

Lead acetate-induced cytotoxicity in Z310 cells

Objective To detect hormesis effects of lead acetate on Z310 cells.Methods Z310 cells were treated with lead acetate at concentrations of 0,0.000 2,0.002,0.02,0.2,2,20,200 and 500 μmol/L for 12 hours and 24 hours.The proliferation viability of Z310 cells was measured by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt(WST-8) assay.And morphological changes in Z310 cells were observed under optical microscope.ResultsLead acetate stimulated cell survival rate(107.06%)at lower concentration(0.02 μmol /L) for 24 hours.Compared with the control,a significant survival rate increase(110.91%,P<0.05) was observed following exposure to 0.2 μmol/L lead acetate for 24 hours,but at higher concentrations(over 2 μmol/L),the survival of the cells was inhibited.The survival rates significantly decreased(79.37% and 76.81%) only at 200 and 500 μmol/L lead acetate for 24 hours tested by MTT(P<0.01).The same effects were observed by WST-8 at the same concentration,with the survival rates of 81.67% and 72.36% for 12 hours(P<0.01) and 56.89% and 44.05% for 24 hours(P<0.01).Conclusion Lead acetate can induce the hormesis of Z310 cell proliferation.There is a higher sensitivity in cell survival rate test with WST-8 than MTT.