DNA－转染体细胞系产生单克隆抗体

本文研究淋巴细胞转染体的产生和转染体单克隆抗体的抗原结合特性．以小鼠宫颈癌细胞系Ｕ１４为抗原免疫小鼠。以制备的Ｕ１４和小鼠骨髓瘤细胞系Ｓｐ２／０ＤＮＡ分别转染经Ｕ１４免疫的小鼠原代脾细胞．将所形成的转染体细胞进行克隆，建成２株淋巴细胞转染体细胞系ＵＤ和ＳＤ．它们能连续生长于含血清和无血清培养液中，但不能在同基因小鼠体内生长．ＵＤ和ＳＤ细胞系所产生的单克隆抗体不仅能与小鼠宫颈癌细胞表面抗原及其可溶性抗原发生特异反应，且能识别人宫颈癌组织和宫颈癌病人血清中的肿瘤抗原决定簇．本实验结果进一步证明了我们提出的肿瘤＂进化抗原＂理论，并提示瘤细胞ＤＮＡ转染造成的淋巴细胞永生化，可能是产生人源性单克隆抗体具有前景的途径．

DNA-Transfectant cell Lines Producing Monoclonal Antibodies

The production of lymphocyte transfectants and antigen-binding characteristics of the monoclonal antibodies from the transfectants were studied.Mouse uterine cervical cancer cell line U14 was used as the antigen to immunize mice. Primed mouse primary spleen cells were transfected with DNA preparations from U14 and mouse myeloma cell line Sp2/0, respectively. The transfectant cells formed were cloned. Two lymphocyte transfectant cell lines UD and SD were established and could be continuously grown in serum-containing media as well as chemically defined seru-free media,but failed to grow in syngeneic mice. Monoclonal antibodies produced by both UD and SD cell lines not only reacted specifically with U14 cell surface antigen and its soluble counterpart, but also recognized the tumor antigenic determinant in both human uterine cervical cancer tissues and the sera from patients with uterine cervical cancers. The results from our experiments lend further support to the tumor"evolutionary antigen"hypothesis we advanced and suggest that transfection by tumor DNA. for rendering lymphocytes immortal might be the promising approach for producing monoclonal antibodies of human origin.