消瘤平移合剂含药血清诱导肝癌H-7402细胞凋亡的实验研究

目的：观察消瘤平移合剂含药血清对肝癌H－7402细胞凋亡的诱导作用。方法：运用血清药理学方法，选择不同浓度的含药血清与人肝癌H－7402细胞共同孵育，于不同时间观察，并与空白血清作对照。结果：消瘤平移合剂含药血清可显著抑制H－7402细胞的增长；H－7402细胞在显微镜下呈现典型的凋亡形态学改变，流式细胞仪检测可见亚G1峰，凋亡呈含药血清浓度、时间依赖性特点，对照组及5％、10％含药血清作用24h，其凋亡率分别为0．49％、5．58％、8．46％，48h凋亡率分别为0．83％、11．58％、14．25％；bcl-2基因检测显示bcl-2基因表达受抑。结论：消瘤平移合剂可通过诱导肿瘤细胞凋亡，发挥其临床抗肿瘤、抗转移的作用。

Experimental Study on Xiaoliu Pingyi Mixture with Medicated Serum in Inducing Apoptosis of Human Hepatocellular Carcinoma Cell Line H-7402

OBJECTIVE: To observe the efficacy of Xiaoliu Pingyi Mixture (XLPY) in inducing the apoptosis of human hepatocellular carcinoma (HCC) cell line H-7402. METHODS: Using serum pharmacologic method, the morphological change of apoptotic cells was observed at different time points by co-culturing with different concentrations of medicated serum containing XLPY with light and fluorescent microscopy, flow cytometry, and immunohistochemical staining methods. Blank serum was taken as control. RESULTS: The different concentration of medicated serum containing XLPY could significantly inhibit the growth of H-7402 cell line in vitro. After treated with medicated serum of XLPY, the cellular morphologic changes were typical apoptosis under light and fluorescent microscopy, the sub-G1 peaks were observed by flow cytometric assays. The apoptosis rate revealed a medicated serum concentration and time dependent manner. The control group 5%, 10% medicated serum induced apoptotic rate were 0.49%, 5.58% and 8.46% by 24 h; while by 48 h, it was 0.83%, 11.58% and 14.25% respectively. bcl-2 gene examination showed that the bcl-2 gene expression was inhibited. CONCLUSION: XLPY could induce apoptosis in carcinoma cells, and play the clinical role of anti-tumor and anti-metastasis.