大黄不同饮片大孔树脂分离部位的提取物总量及其HPLC比较研究

目的:通过对大黄不同饮片大孔树脂分离部位的提取物总量及其HPLC比较,确定饮片炮制前后成分变化明显的组分,探索导致大黄药性变化的药效物质基础。方法:以75%乙醇提取大黄各种饮片,提取物经大孔树脂D101分离,分别制备成水、20%乙醇、50%乙醇和95%乙醇部位,以HPLC对各部位进行比较。结果:饮片总提取物重量由高到低为生片、酒片>醋片>熟片>炭片。酒片、醋片及炭片均为50%乙醇洗脱物最多,生片和熟片均为H₂O洗脱物最多,但生片20%和50%乙醇洗脱物的重量基本一致,而熟片则呈依次递减趋势。各饮片4个洗脱部位中均为95%乙醇洗脱部位的洗脱物最少。HPLC比较结果显示,各组分分离良好,各饮片间的20%和50%及95%乙醇洗脱部位色谱峰变化显著。结论:随着炮制条件的加剧,各饮片化学成分的组成及含量发生了较明显的变化,提取物重量及HPLC比较结果提示,20%,50%及95%乙醇洗脱部位化学成分的变化可能是导致大黄不同饮片药性变化的物质基础。

Comparison on Weight of Macroporous Resin Separation Parts and its HPLC Profile Among Different Chinese Herbal Pieces from Rheum palamatum L.

Objective:To compare the weight of macroporous resin separation parts and its HPLC profile with the different Chinese herbal pieces from Rheum palamatum L., and to explore the effective substance of the processed herbal medicine. Method: 75% ethanol was used for extraction. After the separation by the macroporous resin, four parts were analyzed by HPLC analysis. Result:The total extraction weight from high to low is no-parched, liquored>vinegar-baked>braising with liquor>charred. The mass of 50% ethanol elution part is highest in liquored, vinegar-baked and charred. The 20% and 50% ethanol elution part have the same weight in no-parched, however, the braising with liquor represent a degressive tendency. HPLC results demonstrate that the 20%, 50% and 95%ethanol elution parts differences are remarkably. Conclusion: With the increased processing conditions, the composition of chemical constituents and content in different pieces has obvious changed. Extractions weight and HPLC results indicate that the changes of chemical constituents may result in different property among different Chinese herbal pieces from Rheum palamatum L.