慢性乙型肝炎患者外周血T细胞受体基因重组的意义

目的 通过对慢性乙型肝炎患者外周血Ｔ细胞受体基因β链（Ｔ ｃｅｌｌ ｒｅｃｅｐｔｏｒ ｇｅｎｅ Ｂｅｔａｃｈａｉｎ，ＴＣＲβ）重组与血清ＨＢｅＡｇ，抗－ＨＢｅ和丙氨酸转氨酶（ＡＬＴ）平行检测，以探讨优势Ｔ细胞克隆在病程中的作用。方法 运用Ｓｏｕｔｈｅｒｎ杂交检测８５例慢性乙型肝炎患者外周血单个核细胞ＤＮＡ。结果 ８５例患者ＴＣＲβ重组阳性率为４４．７％，大多数具有ＥｃｏＲⅠ或／和ＨｉｎｄⅢ重组带。

T cell receptor gene rearrangement of Peripheral blood mononuclear cells from patients with chronic hepatitis B

Objective T cell receptor gene (TCR) rearrangement of peripheral blood mononuclear cells from patients with chronic hepatitis B was detected simultaneously with serum HBeAg, anti HBe and alanine aminotransferase (ALT) to examine the role of dominant T cell clones. Methods Southern hybridization was used to detect DNA from peripheral blood mononuclear cells in 85 patients with chronic he patitis B. Results The positive rate of TCR rearrangement was 44.7% in 85 patients with chronic hepatitis B. Most of them demonstrated two kinds of rearrangement bands of DNA fragment. Cross sectional study indicated there was no significant difference in positive rate of HBeAg between TCR positive group and TCR negative group while there was significant difference in ALT between the two groups. Follow up of 19 patients with chronic hepatitis B suggested that appearance of the TCR rearrangement was associated with cytotoxicity to liver and clearance of hepatitis B virus.Conclusion The detection of TCR rearrangement is useful in the evaluation of disease progression and immune response.