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| 透明质酸酶催化透明质酸水解的最适反应条件 | |
| 引用本文： | 崔向珍,刘爱华,王凤山,郭学平.透明质酸酶催化透明质酸水解的最适反应条件[J].中国生化药物杂志,2007,28(3):161-163. |
| 作者姓名： | 崔向珍 刘爱华 王凤山 郭学平 |
| 作者单位： | 1. 山东大学,药学院,生化与生物技术药物研究所,山东,济南,250012;山东省商业职业技术学院生物工程学院,山东,济南,250103 2. 山东省生物药物研究院,博士后工作站,实验室,山东,济南,250108 3. 山东大学,药学院,生化与生物技术药物研究所,山东,济南,250012 |
| 摘 要： | 目的确定透明质酸酶(HAase)催化透明质酸(HA)水解的最适条件。方法HAase不同条件下催化HA水解,反应结束后利用高效凝胶渗透色谱(HPGPC)法测量反应产物的相对分子质量及其分布系数。结果HAase催化HA水解受温度、pH值、酶浓度、底物浓度、反应时间等因素的影响。结论HAase催化HA水解的最适反应条件是底物浓度为10 g/L,酶浓度为150 000 U/L,pH为5.0,反应温度为50℃。 |
| 关 键 词： | 透明质酸 透明质酸酶 水解 高效凝胶渗透色谱 |
| 文章编号： | 1005-1678（2007）03-0161-03 |
| 收稿时间： | 2006-07-17 |
| 修稿时间： | 2006-09-25 |
Reaction conditions of hyaluronic acid hydrolysis catalyzed by hyaluronidase |
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| CUI Xiang-zhen,LIU Ai-hua,WANG Feng-shan,GUO Xue-ping.Reaction conditions of hyaluronic acid hydrolysis catalyzed by hyaluronidase[J].Chinese Journal of Biochemical Pharmaceutics,2007,28(3):161-163. | |
| Authors: | CUI Xiang-zhen LIU Ai-hua WANG Feng-shan GUO Xue-ping |
| Institution: | 1. Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Science, Shangdong University, Jinan 250012, China; 2. College of Bio-engineering , Shandong Institute of Commerce and Technology, Jinan 250103, China ; 3. Institute of Biopharmaceuticals of Shandong Province, Jinan 250108, China |
| Abstract: | Purpose To study the optimal reaction conditions of hyaluronic acid(HA) hydrolysis catalyzed by hyaluronidase(HAase).Methods Hyaluronic acid was hydrolyzed by hyaluronidase catalyzation under different conditions,and the relative molecular weight(Mr) and the Mr distribution of the product(PD value)was studied by high performance gel permeation chromatography(HPGPC).Results The hydrolysis reaction is affected by temperature,pH,substrate concentration,the amount of HAase used and the reaction time.Conclusion The optimal reaction conditions are as follows:10 g/L of HA concentration,150 000 U/L of HAase,pH 5.0,and reaction temperature 50℃. |
| Keywords: | hyaluronic acid hyaluronidase hydrolysis HPGPC |
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