| 酶联免疫吸附试验在微囊藻毒素检测中的应用进展 |
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| 引用本文： | 何小维,郭秋华,彭运平,黄强.酶联免疫吸附试验在微囊藻毒素检测中的应用进展[J].卫生研究,2007,36(3):388-390. |
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| 作者姓名： | 何小维 郭秋华 彭运平 黄强 |
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| 作者单位： | 1. 华南理工大学轻工与食品学院,广州,510640
2. 广州万孚生物技术有限公司 |
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| 摘 要： | 微囊藻毒素(MCs)是蓝绿藻属产生的一类密切相关的环状七肽毒素,其毒性主要表现在特异性抑制细胞内的蛋白磷酸酶(分别为PP1和PP2A)。目前有关微囊藻毒素分析检测的研究方法有高效液相色谱(HPLC)、质谱(LC-MS)、蛋白磷酸酶抑制试验(PPIA)和酶联免疫吸附试验(ELISA)等,本文主要介绍了应用酶联免疫吸附法检测MCs的研究进展,并对ELISA在天然水中检测微囊藻毒素的应用前景进行了展望。
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| 关 键 词： | 蓝藻水华 微囊藻毒素 单克隆抗体 酶联免疫吸附试验 |
| 文章编号： | 1000-8020（2007）03-0388-03 |
| 修稿时间： | 2006-10-10 |
Application of ELISA for microcystins detection |
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| HE Xiaowei,GUO Qiuhua,PENG Yunping,HUANG Qiang.Application of ELISA for microcystins detection[J].Journal of Hygiene Research,2007,36(3):388-390. |
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| Authors: | HE Xiaowei GUO Qiuhua PENG Yunping HUANG Qiang |
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| Institution: | College of Light Industy and Food Science, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | Microcystins (MCs) may be group of closely related toxic cyclic heptapeptides produced by common cyanobacteria(blue-green algae). Their toxicity could be associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). There are some methods for analyzing and detecting MCs in aquatic environment, such as HPLC, LC-MS, PPIA, and ELISA. This review introduces advances on MCs determined by enzyme-linked immunosorbent assay (ELISA), and estimates the prospective development of ELISA for monitoring MCs in natural waters. |
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| Keywords: | cyanobactefial blooms microcystins monoclonal antibodies ELISA |
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