Localization of the mRNA for the 5-HT2 receptor by in situ hybridization histochemistry. Correlation with the distribution of receptor sites |
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| Authors: | Guadalupe Mengod, Maria Pompeiano, M. Inocencia Marti nez-Mir,Jose M. Palacios |
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| Affiliation: | Guadalupe Mengod, Maria Pompeiano, M. Inocencia Marti´nez-Mir,Jose´M. Palacios |
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| Abstract: | 32P-labelled oligonucleotides complementary to rat 5-HT2 receptor mRNA were used as probes to study the distribution of cells in rat brain containing the mRNA coding for this receptor by in situ hybridization histochemistry. 5-HT2 receptor binding sites were visualized by autoradiography using [125I]DOI as ligand. Both distributions were comparable, demonstrating that 5-HT2 receptors are expressed by cells intrinsic to the neocortex (lamina Va), claustrum, olfactory bulb and several nuclei of the brainstem. |
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| Keywords: | Serotonin-2 receptor In situ hybridization Oligonucleotide mRNA Receptor autoradiography Rat brain |
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