免疫层析试纸条诊断旋毛虫病的研究

目的建立一种能诊断人体旋毛虫病及动物旋毛虫感染的快速血清学方法。方法以胶体金标SPA和旋毛虫肌幼虫ES抗原制备免疫层析试纸条(immunochromatographic strip),对旋毛虫病及其它寄生虫病患者血清、旋毛虫及其它寄生虫感染的动物血清进行检测。结果试纸条检测旋毛虫病患者与旋毛虫感染的小鼠、大鼠、兔、猪血清的阳性率分别为100%(20/20)、97.87%(92/94)、100%(5/5)、100%(5/5)及100%(25/25),15min内肉眼可观察结果;其它寄生虫病(并殖吸虫病、血吸虫病、华支睾吸虫病、囊虫病及包虫病等)患者及正常人血清、其它寄生虫感染动物及正常动物血清均为阴性。试纸条和ELISA对100条幼虫感染小鼠血清检测的阳性率分别为91.3%(21/23)和95.7%(22/23)(χ2=0.36,P>0.05),两种方法对200～500条幼虫感染小鼠血清检测的阳性率均为100%(71/71)。试纸条对乡土旋毛虫、布氏旋毛虫、伪旋毛虫及纳氏旋毛虫感染小鼠血清检测的阳性率亦均为100%。试纸条在4℃可保存13个月,检测结果在室温可保存3个月。结论该试纸条可用于人体旋毛虫病和动物旋毛虫感染的快速血清学诊断,也可用于其它种旋毛虫感染的血清流行病学调查。

Immunochromatographic strip for diagnosis of trichinellosis

To establish one rapid serological method for diagnosis of human tricinellosis and animal Trichinella infection,the immunochromatographic strip was prepared by using the SPA labeled with colloidal gold and T.spiralis muscle larval excretory-secretory(ES) antigens.Anti-Trichinella antibodies in sera from patients with trichinellosis or other parasitosis,and from animals infected with Trichinella or other parasites were assayed by this strip.The serum antibody positive rate of patients with trichinellosis,and mice,rats,rabbits,pigs infected with T.spiralis was 100%(20/20),97.87%(92/94),100%(5/5),100%(5/5)and 100%(25/25),respectively.The results could be observed by naked eyes within 15 min.All sera from patients with other parasitosis(paragonimiasis,schistosomiasis,clonorchiasis,cysticercosis,echinococcosis,etc.) as well as,normal persons,animals infected with other parasites,and normal animals showed negative results.When sera from mice infected with 100 T.spiralis larvae were assayed,the positive rate of the strip and ELISA was 91.3%(21/23) and 95.7%(22/23),respectively(χ2=0.36,P﹥0.05).However,the positive rate of both methods was 100%(71/71) when sera from mice infected with 200-500 larvae were assayed.The serum antibody positive rate of mice infected with T.nativa(T2),T.britovi(T3),T.pseudospiralis(T4) and T.nelsoni(T7) was also 100%.This strip could be conserved at 4℃ for 13 months and the results at room temperature for 3 months.These results suggest that the strip could be used for the rapid serodiagnosis of human trichinellosis and animal T.spiralis infection,and the seroepidemi logical investigation of Trichinella spp.infection.