| HPLC法检测蛇莓中齐墩果酸的含量 |
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| 引用本文： | 袁晓环,王伟光,李春彦,武艳,张羽飞,初彦辉.HPLC法检测蛇莓中齐墩果酸的含量[J].中国生化药物杂志,2010,31(3). |
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| 作者姓名： | 袁晓环 王伟光 李春彦 武艳 张羽飞 初彦辉 |
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| 作者单位： | 1. 牡丹江医学院,黑龙江省抗纤维化生物治疗重点实验室,黑龙江,牡丹,157011
2. 牡丹江医学院,药学系,黑龙江,牡丹,157011 |
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| 摘 要： | 目的探讨HPLC测定蛇莓中齐墩果酸含量的方法。方法采用Thermo ODS-2HYPERTIL C18(250 mm×4.6 mm,5μm)色谱柱;流动相:0.065 moL/L NaH2PO4溶液-甲醇(14∶86);检测波长:210 nm;流速:0.8 mL/min。结果齐墩果酸在0.19～2.16μg范围内呈良好线性关系(r=0.998 2);平均回收率为98.6%,RSD(n=9)为1.68%。结论此法简单、准确、可靠,可以作为评价蛇莓质量的有效方法。
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| 关 键 词： | 蛇莓 齐墩果酸 高效液相色谱 |
Determination of oleanolic acid in Duchesnea Indicae by HPLC method |
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| YUAN Xiao-huan,WANG Wei-guang,LI Chun-yan,WU Yan,ZHANG Yu-fei,CHU Yan-hui.Determination of oleanolic acid in Duchesnea Indicae by HPLC method[J].Chinese Journal of Biochemical Pharmaceutics,2010,31(3). |
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| Authors: | YUAN Xiao-huan WANG Wei-guang LI Chun-yan WU Yan ZHANG Yu-fei CHU Yan-hui |
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| Abstract: | Purpose To investigate the method about the determination of oleanolic acid in Duchesnea Indicae.Methods The chromatographic separation was performed on a Thermo ODS-2HYPER-TIL C18(250 mm×4.6 mm,5 μm).The column temperature was 25 ℃.The mobile phase consists of NaH2PO4(0.065 mol/L)-Methyl alcohol(14∶86).Examination wave length was 210 nm.The flow rate was 0.8 mL/min.Results The liner relationship of oleanolic acid was good within the range of 0.19-2.16 μg(r=0.998 2).The average recovery was 98.6% and RSD(n=9) was 1.68%.Conclusion The method is simple,accurate and reliable,and it can be used as an effective way to evaluate the quality of Duchesnea. |
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| Keywords: | Duchesnea Indicae oleanolic acid HPLC |
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