肾移植受者中CMV感染的检测

目的探讨巨细胞病毒(Cytomegalovirus,CMV)在肾移植受者中的感染状况.方法应用酶联免疫吸附试验(ELISA)、免疫组化方法及聚合酶链反应技术测定167例肾移植受者的CMV抗体、抗原和CMVDNA.结果肾移植受者的抗CMVIgG和抗CMVIgM阳性率分别为98.8%和1.8%;CMV抗原阳性细胞数平均为(3.2±3.1)/5×104WBC,阳性率为47.3%;CMVDNA的阳性率为50.9%.结论肾移植受者术后存在不同程度的CMV感染.临床上开展CMV抗体、抗原及核酸的检测对早期诊断肾移植受者术后CMV感染具有重要意义.

Detection of cytomegalovirus infection in recipients of renal transplantation

OBJECTIVE: To investigate infection of cytomegalovirus (CMV) in recipients of renal transplantation (RT). METHODS: 167 recipients of RT were screened for the occurrence of CMV infection by using ELISA to test anti-CMV IgG, IgM from serum samples, using immunohistochemistry method to test CMV antigen expression and nest PCR to amplify CMV DNA from blood samples. RESULTS: Of these samples, 165 and 3 came out positive of IgG and IgM with the positive rate being 98.8% and 1.8% respectively. 79 samples resulted in antigen expression (47.3%) with the average antigen index being (3.2, 3.1)/5, 104 WBC. Besides, 85 samples were found to be positive by nest-PCR with the positive rate being 50.9%. CONCLUSIONS: The results indicate that the infection of RT so the detection CMV is common in recipients of RT. So detection of CMV-antibody, CMV antigen and CMV DNA after renal transplantation is very important in early diagnosis of CMV infection.