酪酪肽对荷人肝癌裸鼠皮下移植瘤的影响

目的 研究酪酪肽(PYY)对荷人肝癌裸鼠皮下移植瘤生长的影响并初步探讨其作用机制.方法 用含1×107/ml的HepG2人肝癌细胞悬液0.2 ml打人裸鼠皮下,待其长出的肿瘤直径约1cm时将瘤块取出,在无菌PBS液中剪成大小1 min×1 mm×1mm的小块,用组织插块法制作裸鼠皮下肝癌模型.选取成瘤的32只裸鼠平均分为4组:PYY大、小剂量组,阳性对照组给与腹腔注射氟尿苷,阴性对照组给与皮下注射生理盐水.实验过程中观察裸鼠的一般情况.结果 PYY大、小剂量组在肿瘤体积、血清AFP、瘤重及瘤组织cAMP中均低于阴性对照组(P＜0.05).结论 PYY可以抑制裸鼠肝癌皮下移植瘤的生长,其机制可能与抑制肝癌细胞内cAMP的含量有关.

Effect of peptide YY on subcutaneous transplantation tumor of human hepatoma in nude mice

OBJECTIVE:To investigate the effects of peptide YY (PYY) on subcutaneous transplantation tumor of human hepatoma in nude mice and preliminarily explore the mechanisms. METHODS: HepG2 human hepatic carcinoma cells were injected into nude mice subcutaneously, and the resultant tumor were taken and prepared into small tissue blocks. The tissue blocks were implanted subcutaneously into nude mice to establish mouse models bearing human hepatoma. Thirty-two such mouse models were assigned equally into 4 groups to receive subcutaneous PYY injection at a high or low dose, intraperitoneal injection of floxuridine (positive control group), or subcutaneous normal saline injection (negative control group). The general condition of the tumor-bearing mice and the growth of the tumors were observed. RESULTS: Compared with the negative control group, the high- and low-dose PYY groups showed reduced gross tumor volume, lowered serum AFP, tumor weight, and cAMP content in the tumor tissue (P<0.05). CONCLUSION: PYY can inhibit the growth of subcutaneous hepatoma in nude mice, which might be associated with the reduction of cAMP content in the tumors following PYY treatment.