大肠癌组织中VEGF-C表达与淋巴管生成及临床意义

[目的]探讨大肠癌组织中血管内皮生长因子-C（VEGF-C）、淋巴管密度（LMVD）的表达及其与临床病理参数的关系。[方法]对50例大肠癌组织、正常肠组织分别采用5′-Nase-酶组织化学技术及免疫组化SP法测定LMVD和VEGF-C。[结果]VEGF-C阳性组中的LMVD高于阴性组（11．86±2．42VS．7．82±2．69，P〈0．01），大肠癌LMVD与VEGF-C的表达呈正相关，大肠癌LMVD与VEGF-C的表达与淋巴转移相关（P〈0．01）。[结论]VEGF-C的过表达可促进淋巴管生成，导致LMVD的升高，进而促进大肠癌淋巴转移，LMVD与VEGF-C可作为大肠癌淋巴转移的预测因子。

Expression of VEGF-C and Lymphangiogenesis in Colorectal Carcinoma and Its Significance

[Purpose] To study the expression of vascular endothelial growth factor C（VEGF-C） and lymphatic microvessel density（LMVD） in colorectal carcinoma, and its correlation with clinicopatho- logical parameters. [ Methods ] The expressions of VEGF-C and LMVD were detected by S-P immunohistochemical staining and by 5′-nucleotidase-enzyme-histochemical staining respectively in 50 specimens of colorectal carcinoma tissues and normal colorectal tissues. [Results] In VEGF-C positive group LMVD was significantly higher than that in VEGF-C negative group （11.86±2.42 vs 7.82±2.69, P〈0.01）. LMVD was positively correlated with the expression of VEGF-C. VEGF-C and LMVD were correlated with lymphatic metastasis in colorectal carcinoma （P〈0.01）. [Conclusion ] Overexpression of VEGF-C promoting lymphangiogenesis results in LMVD increasing, lymphatic metastasis in colorectal carcinoma. LMVD and VEGF-C may act as predictors of lymphatic metastasis.