| 大鼠皮层神经元原代培养方法的改进 |
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| 引用本文： | 余华荣,杨贵忠,杨俊卿,周岐新,汤为学. 大鼠皮层神经元原代培养方法的改进[J]. 重庆医科大学学报, 2007, 32(4): 392-394,401 |
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| 作者姓名： | 余华荣 杨贵忠 杨俊卿 周岐新 汤为学 |
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| 作者单位： | 重庆医科大学基础医学院生理教研室,重庆,400016;重庆医科大学药学院药理教研室,重庆,400016;重庆医科大学基础医学院病理生理教研室,重庆,400016 |
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| 摘 要： | 目的:建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法.方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养.结果:在体外培养条件下神经元结构特征明显,并能形成典型的神经元网络.结论:本方法适合普通实验室进行大鼠皮层神经元体外培养.
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| 关 键 词： | 细胞 培养的 神经系统 大鼠 |
| 文章编号： | 0253-3626（2007）04-0392-03 |
| 修稿时间： | 2007-01-09 |
Improvement for culture of primary neurons of newborn rat cortical tissue |
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| YU Huarong,et al. Improvement for culture of primary neurons of newborn rat cortical tissue[J]. Journal of Chongqing Medical University, 2007, 32(4): 392-394,401 |
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| Authors: | YU Huarong et al |
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| Affiliation: | Department of Physiology, College of Basic Medicine, Chongqing Medical University |
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| Abstract: | Objective:To establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tissure. Methods:Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. Results:Under the culture condition in vitro,newborn rat neurons showed similar properties to their counterparts in vivo and typical nerver fiber net was formed. Conclusion:This technique for the primary culture of cortical neurons is an ideal good technique for the general labs. |
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| Keywords: | Cells Cultured Nervous system Rats |
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