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| 成骨细胞对葡萄糖水平的感知作用 | |
| 引用本文： | 赵红莉,赵红秋,傅晓英,蒙荣森,张莹,严励,程桦,徐明彤,张少玲,傅祖植.成骨细胞对葡萄糖水平的感知作用[J].中国组织工程研究与临床康复,2010,14(2). |
| 作者姓名： | 赵红莉 赵红秋 傅晓英 蒙荣森 张莹 严励 程桦 徐明彤 张少玲 傅祖植 |
| 作者单位： | 1. 中山大学附属第二医院内分泌科,广东省广州市,510120;广东省第二人民医院分泌科,广东省广州市,510317 2. 山西医科大学第一医院检验科,山西省太原市,030001 3. 广东省第二人民医院分泌科,广东省广州市,510317 4. 中山大学附属第一医心血管医学部,广东省广州,510120 5. 广州医学院第三附属院内分泌科,广东省广州,510150 6. 中山大学附属第二医院内分泌科,广东省广州市,510120 |
| 摘 要： | 背景:新近研究表明,骨钙素可提高胰岛素分泌水平、胰岛素敏感性,并且可阻止脂肪的积累,参与糖脂代谢,发挥此作用的主要是羧化不全骨钙素.目的:观察不同浓度葡萄糖对成骨细胞羧化不全骨钙素分泌水平的影响.方法:切下人肋骨骨小梁剪碎,胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞,以DMEM完全培养液清洗骨小梁1次,移入培养瓶内培养,每周更换培养液1次,1周后可见成骨细胞自骨片移出,4～6周细胞融合成单层可作传代培养.取生长活跃的传二三代细胞接种于6孔板中分成5组,待细胞融合至80%以含5.6,7.6,9.6,12.6,20.6 mmol/L葡萄糖的培养基刺激成骨细胞,同时加入维生素K_2使其在培养液中最终浓度为10~(-5)mol/L.培养半小时后收集上清液,以放免法检测成骨细胞培养上清液中羧化不全骨钙素水平,并以上清液中总骨钙素进行校正,计算羧化不全骨钙素率.结果与结论:在不同葡萄糖浓度下成骨细胞羧化不全骨钙素分泌水平不同,7.6,9.6,20.6 mmol/L葡萄糖浓度组羧化不全骨钙素率高于5.6 mmol/L葡萄糖浓度组(0.27±0.02),(0.29±0.04),(0.12±0.02)%,P<0.05].提示成骨细胞可感知葡萄糖浓度变化,在5.6～9.6mmol/L葡萄糖刺激下成骨细胞可呈剂量依赖性地提高自身羧化不全骨钙素分泌水平,进一步提高葡萄糖浓度时羧化不全骨钙素水平反而降低. |
| 关 键 词： | 成骨细胞 骨钙素 羧化不全骨钙素 葡萄糖 骨组织工程 |
Role of osteoblasts in the sense of glucose |
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| Zhao Hong-li,Zhao Hong-qiu,Fu Xiao-ying,Meng Rong-sen,Zhang Ying,Yan Li,Cheng Hua,Xu Ming-tong,Zhang Shao-ling,Fu Zu-zhi.Role of osteoblasts in the sense of glucose[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(2). | |
| Authors: | Zhao Hong-li Zhao Hong-qiu Fu Xiao-ying Meng Rong-sen Zhang Ying Yan Li Cheng Hua Xu Ming-tong Zhang Shao-ling Fu Zu-zhi |
| Institution: | Zhao Hong-li1,2,Zhao Hong-qiu3,Fu Xiao-ying2,Meng Rong-sen4,Zhang Ying5,Yan Li1,Cheng Hua1,Xu Ming-tong1,Zhang Shao-ling1,Fu Zu-zhi11Department of Endocrinology,Second Affiliated Hospital of Sun Yat-sen University,Guangzhou 510120,Guangdong Province,China,2Department of Endocrinology,No.2 Guangdong Provincial People\'s Hospital,Guangzhou 510317,3Laboratory Center,First Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi Province,4Department of Cardiology,... |
| Abstract: | BACKGROUND:Recent study showed that osteocalcin may elevate Insulin secretion and sensitivity,prevent the fat accumulation,play a role in the metablism of glucose and lipid. Undercarboxylated osteocalcin works as the main role. OBJECTIVE:To investigate the effect of different concentrations of glucose on osteoblast undercarboxylated osteocalcin. METHODS:The rib trabeculae were resected and broken,trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were ... |
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