张力作用下牙周组织破骨细胞分化因子、破骨细胞发生抑制因子表达变化的研究

目的:运用原位杂交的方法,观察破骨细胞分化因子(ODF)、破骨细胞发生抑制因子(OCIF)在正畸大鼠牙周组织改建过程中张力侧的表达变化,探讨ODF、OCIF与正畸牙周组织改建的关系。方法:8周龄成年健康雄性SD大鼠30只,随机分为对照组、牙齿移动1d组、3d组、5d组、7d组,共5组,每组6只。在大鼠上颌右侧第一磨牙与上颌切牙之间安置正畸矫治器装置,施力50g。在相应时间段处死实验动物,取材固定,进行原位杂交染色、图像分析。结果:在牙齿移动3d后,OCIF原位杂交染色在张力侧逐渐深染,OCIF mR-NA阳性细胞数量逐渐增多,OCIF阳性表达在5d时达到最高,并可见到有新骨形成;7d时OCIF阳性表达及分布情况与3d时相类似。张力侧牙周膜细胞、成骨细胞的ODF原位杂交染色阳性反应随天数的增加有逐渐增强趋势,并可观察到有ODFmRNA阳性破骨细胞出现,但表达变化并不明显。结论:ODF及OCIF参与了正畸牙周组织改建过程,OCIFmRNA在张力区随正畸牙齿移动表达升高具有时间依赖性。

The expression of ODF and OCIF by periodontal tissue under strain force in rats

AIM:To investigate the changes of ODF and OCIF expression by periodontal tissue during orthodontic tooth movement in rats and detect the relationship between ODF, OCIF and orthodontic periodontal tissue remodeling. METHODS: Orthodontic appliance was placed between the right maxillary first molar and maxillary central incisors of rat. Thirty rats were divided into 5 groups: the 1-day, 3-day, 5-day, 7-day after application of orthodontic force and control group. Then in situ hybridization and image analyses were performed. RESULTS: The expression of ODF and OCIF was weak in normal periodontal tissue; in strain area,OCIF expression increased in 3 days,reached the maximum at day 5,new bone formation could be seen at that time; ODF expression decreased during orthodontic tooth movement but not significantly. CONCLUSION: ODF and OCIF participated in orthodontic periodontal tissue remodeling. OCIF expression increased in strain area in a time-dependent manner.