沉默ABCG2基因对人肺腺癌细胞GLC-82体内增殖影响的定量研究

目的探讨ABCG2基因沉默对人肺腺癌细胞GLC-82体内增殖的影响。方法将人肺腺癌细胞GLC-82、绿色增强荧光蛋白（EGFP）标记的细胞GLC-82／EGFP^＋与ABCG2基因沉默的细胞GLC-82／EGFP^＋／ABCG2^-分别接种于裸鼠皮下，用免疫组化SP法及银染法，检测成瘤后瘤组织中细胞增殖指数Ki-67的变化与核仁组成区嗜银蛋白（AgNOR）在瘤细胞中的变化，测试肿瘤细胞AgNOR颗粒截面数、单个AgNOR颗粒面积、单个核AgNOR总面积，并用图像分析系统对ABCG2蛋白在瘤细胞中的表达进行定量分析。结果裸鼠体内GLC-82／EGFP^＋／ABCG2^-细胞成瘤组织与GLC-82、GLC-82／EGFP^＋细胞成瘤组织相比，前者ABCG2蛋白的表达明显减弱（P〈0．05），Ki-67增殖指数显著降低（P〈0．05）且瘤细胞AgNORs颗粒截面数、单个核内AgNOR总面积明显减少，而单个AgNORs颗粒面积增大，差异有显著性（P〈0．05）。结论沉默ABCG2基因能抑制人肺腺癌细胞GLC-82在体内的增殖。

Quantitative study on the inhibitory effect of ABCG2 gene silencing on the proliferative ability of human lung carcinoma cells in vivo

Objective To observe the effect of ABCG2 gene silencing on proliferative ability in vivo of human lung carcinoma cells. Methods Human lung carcinoma cell line GLC-82, enhanced green fluorescence protein （EGFP）-labeled human lung carcinoma cells GLC-82/EGFP^＋ and GLC-82/EGFP^＋/ ABCG2^- were inoculated into subcutaneous tissue in nude mice. Expressions of Ki-67, ABCG2 and AgNOR in the tumors were examined by immunohistochemistry and quantitative image analysis. Results The Ki-67 proliferation index of GLC-82/EGFP^＋/ABCG2^- cells in vivo was significantly lower than that of GLC-82/EGFP^＋ cells and GLC-82 cells （P 〈 0.05） , as well as the expression of ABCG2 protein. Image analysis revealed significantly lesser number of AgNOR and total AgNOR area but higher mean AgNOR area in the cells of GLC-82/EGFP^＋/ABCG2^- group. Conclusion ABCG2 gene silencing can suppress the proliferative ability of lung carcinoma cells in vivo.