石蜡包埋组织自动和手动切片对切片厚度的影响

目的观察手动与自动两种切片方法和组织种类对实测切片厚度的影响。方法选取5只成年健康雄性SD大鼠的肝、胰腺、胃及肺4种组织，各组织单独包埋制成蜡块，预设电动切片机的切片厚度刻度值为3、5、7、10μm，分别作自动和手动两种方法切片，所获得的薄组织片经二次包埋、切片、HE染色，TIGER图像分析仪测量切片的实际厚度。结果组织切片实测厚度受自动和手动两种切片方法的影响，切片机的切片厚度预设值低估了石蜡包埋组织切片的真实厚度，自动和手动实测切片厚度分别比切片机预设切片厚度要厚29％～50％和48％～91％。结论自动切片与手动切片的切片厚度相差25％，二者相对误差为61％，自动切片获得的组织切片的厚度精度较高，优于手动切片。

Effect of automatic and manual slicing on thickness variation of paraffin-embedded sections

Objective To observe the effect of automatic and manual slicing and tissue types on the thickness variation of paraffin-embedded sections. Methods Liver, pancreas, stomach and lung samples were obtained from five healthy adult male rats. Each tissue was separately embedded in paraffin. Slices were cut automatically and manually at various thickness set points, 3,5 ,7,10 μm, by an automatic microtome. After the re-embedding vertical slicing and HE staining, the thickness of these slices was measured by a TIGER image analysis system. Results Only the automatic or manual slicing procedure exerted effect on the slice thickness. The slice thickness set points of microtome underestimated the actual thick- ness of paraffin-embedded slices. The thickness of auto-slice and manual slice were 29% - 50% and 48 % - 91% , respectively, thicker than the is a 25% difference between the thicknesses tive error rate of the two slicing methods is nominal thickness set on the microtome. of automatically cut slices and manually 61%. The precision of automatic cut Conclusion There cut slices. The relathickness is higher,therefore, is better than manual sectioning.