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| 蛇毒纤溶酶的分离纯化及其溶栓作用 | |
| 引用本文： | 马骉,张骞,宋梦薇,吴丹,林琳,付学奇,林永齐. 蛇毒纤溶酶的分离纯化及其溶栓作用[J]. 中国生化药物杂志, 2004, 25(3): 156-158,180 |
| 作者姓名： | 马骉 张骞 宋梦薇 吴丹 林琳 付学奇 林永齐 |
| 作者单位： | 1. 北京赛生药业有限公司,北京,100176 2. 吉林大学,生命科学学院,吉林,长春,130023 3. 大连医科大学,生化室,辽宁,大连,116027 |
| 摘 要： | 目的获得大量高纯度的蛇毒纤溶酶并进行药效实验。方法用离子交换柱色谱和单克隆抗体亲和色谱技术进行纯化 ,用体外和半体内血栓模型研究其抗血栓作用。结果得到纯度为 95 %以上的纤溶酶。结论纯化后的蛇毒纤溶酶具有显著的抑制血栓形成的作用 |
| 关 键 词： | 蛇毒 纤溶酶 溶栓 亲和色谱 |
| 文章编号： | 1005-1678(2004)03-0156-03 |
Purification of fibrinoenase from snake venom and its fibrinolytic effect |
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| MA Biao ,ZHANG Qian ,SONG Meng wei ,WU Dan ,LIN Lin ,FU Xue qi ,LIN Yong qi. Purification of fibrinoenase from snake venom and its fibrinolytic effect[J]. Chinese Journal of Biochemical Pharmaceutics, 2004, 25(3): 156-158,180 | |
| Authors: | MA Biao ZHANG Qian SONG Meng wei WU Dan LIN Lin FU Xue qi LIN Yong qi |
| Affiliation: | MA Biao 1,ZHANG Qian 2,SONG Meng wei 1,WU Dan 1,LIN Lin 3,FU Xue qi 2,LIN Yong qi 2 |
| Abstract: | PurposeThe aim is to establish a new method for purification of the fibrinoenase from snake venom by affinity chromatography.MethodsMonoclonal antibody affinity chromatograpy was used. ResultsThe purity of fibrinoenase of above 95% by HPLC was obtained.ConclusionThe purified method was efficient for purifying fibrinoenase.The results indicate that the fibrinoenase had valuable fibrinolytic effect. |
| Keywords: | snake venom fibrinoenase fibrinolytic effect affinity chromatography |
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