血管紧张素转换酶和血管紧张素Ⅱ受体1A1166C基因多态性与脑出血的关系

目的探讨血管紧张素转换酶（ACE）基因插入/缺失多态性和血管紧张素Ⅱ受体1（AT1R）A1166C基因多态性与脑出血的关系,并分析两者是否有协同致脑出血的效应。方法应用聚合酶链反应及限制性片段长度多态性技术,检测80例脑出血患者（脑出血组）和90名健康对照者（健康对照组）的ACE和AT1R基因型和等位基因,并运用Logistic回归分析不同基因型致脑出血的效应。结果脑出血组ACEDD基因型频率为35.0%,D等位基因频率为51.9%,明显高于健康对照组的15.6%和38.9%,均P〈0.05;AT1RAC基因型频率为32.5%,C等位基因频率为16.2%,明显高于健康对照组的11.1%和5.6%,均P〈0.05;Logistic回归显示,170例入组者中,携带AT1RAC基因型（OR：3.852,95%CI：1.719～8.632;P〈0.01）、ACE基因DD型（OR：2.923;95%CI：1.406～6.079;P〈0.01）及同时携带有AT1RAC基因型和ACEDD基因型（OR：4.250;95%CI：1.479～12.209;P〈0.01）是脑出血的独立危险因素。结论ACE基因插入/缺失多态性和AT1RA1166C基因多态性可能是脑出血发病的独立遗传因素;且两者间具有协同致脑出血的作用。

Relationship between angiotensin I-converting enzyme and angiotensin Ⅱ type 1 receptor A1166C gene polymorphisms and intracerebral hemorrhage

Objective To investigate the relationship between angiotensin I-converting enzyme(ACE)gene insertion/deletion and angiotensin II type 1 receptor(AT1R)A1166C gene polymorphisms and intracerebral hemorrhage(ICH)and to analyze whether both of them had the synergistic effect in causing ICH.Methods ACE and AT1R genotypes and alleles were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RLFP)in 80 patients with ICH and 90 healthy controls,and the effect of different genetypes in causing ICH were analyzed by Logistic regression analysis.Results The ACE DD genotype frequency was 35.0%,and the D allele genotype frequency was 51.9% in the ICH group.It was significantly higher than 15.6% and 38.9% in the healthy control group(P<0.05 all);the AT1R AC genotype frequency was 32.5%,and the C allele genotype frequency was 16.2%.It was significantly higher than 11.1% and 5.6% in the healthy control group(P<0.05 all);Logistic regression showed that among the 170 recruited subjects who carried AT1R AC genetype(OR:3.852,95% CI,1.719-8.632;P<0.01),ACE DD genetype(OR:2.923;95% CI,1.406-6.079;P<0.01),and the subjects who carried both AT1R AC and ACE DD genotypes(OR:4.250;95% CI,1.479-12.209;P<0.01)were the independent risk factors for ICH.Conclusion ACE gene insertion/deletion polymorphisms and AT1R A1166C gene may be an independent genetic factor for the onset of ICH,and there were synergistic effect in causing ICH between them.