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Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana |
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| Authors: | Alexandre de Andrade Fabio Siviero Paula Rezende-Teixeira Roberto Vicente Santelli Glaucia Maria Machado-Santelli |
| Affiliation: | 1. Instituto de Química, Departamento de Bioquímica, Universidade de S?o Paulo, Av. Prof. Lineu Prestes 748, S?o Paulo, SP, 05508-900, Brazil 2. Instituto de Ciências Biomédicas, Departamento de Biologia Celular e Desenvolvimento, Universidade de S?o Paulo, Av. Prof. Lineu Prestes 1524, S?o Paulo, SP, 05508-900, Brazil 3. Departamento de Biologia Celular e do Desenvolvimento, Universidade de S?o Paulo, Av. Prof. Lineu Prestes 1524, S?o Paulo, SP, 05508-900, Brazil |
| Abstract: | An hsc70 homologue gene (Rahsc70) of the diptera Rhynchosciara americana was isolated and characterized. We were able to determine the mRNA sequence from an EST of salivary gland cDNA library, and a Rahsc70 cDNA cassette was used as a probe to isolate the genomic region from a genomic library. The mRNA expression of this gene parallels the 2B puff expansion, suggesting its involvement in protein processing, since this larval period corresponds to a high synthetic activity period. During heat shock stress conditions, hsc70 expression decreased. In situ hybridization of polytene chromosomes showed that the Rahsc70 gene is located near the C3 DNA puff. The cellular localization of Hsc70 protein showed this protein in the cytoplasm and in the nucleus. |
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