实验性重症型粪类圆线虫病

以人源粪类圆线虫接种四只幼狗均感染成功,从接种到粪便现蚴为11～13天。当粪便现蚴后一周给予其中三只狗每天5mg/kg强的松口服作免疫抑制处理,一只作对照。免疫抑制处理后两周三只狗腹泻加重,排蚴量明显增加,每克粪便排蚴量达3500～5000条时动物很快死亡。解剖动物发现成虫主要寄生于十二指肠和空肠。病理学检查均有溃疡性十二指肠炎、空肠炎、结肠炎以及肺炎和肺出血。本研究三只幼狗给予免疫抑制后引起重症粪类圆线虫病,其寄生虫学及病理学检查结果和临床表现与人体重症粪类圆线虫病极为相似,可作为暴发性粪类圆线虫病感染的实验室模型。

EXPERIMENTAL SEVERE FORM OF STRONGYLOIDIASIS

Four young dogs were successfully infected with filariform larvae(500 to 1000 for each dog)isolated from patients with strongyloidiasis, and larvae appeared in thier stool 11 to 13 days after inoculation.One week after patent infection three dogs given orally prednisone at a daily dose of 5mg/kg body weight,and the another one served as control.Two weeks after prednisone treatment all the 3 dogs showed aggravated diarrhea and marked increase in the larval output in their stool.The animals rapidly came to death when the larval output rose to 3500 to 5000 per gram of feces.On aoutopsy it was found that the adult worms resided mainly in duodenum and jejunum. Pathological examination revealed marked ulcerative duodenitis,jejunitis,colitis, pnumonia and pulmonary haemorrage.In the light of the fact that prednisone- treated puppies,while infected with S.stercoralis,showed very similar paras- itological,pathological and clinical features as in human beings,they might be used as laboratory model for fulminating strongyloidiasis infection.