广东人群CYP1A1 MspⅠ基因多态性与肺癌易感性研究

目的：探讨广东人群CYP1A1 MspⅠ基因多态性与肺癌易感性的关系。方法：以病例-对照的研究方法，采用PCR技术，检测原发性肺癌患者、住院对照各91例及47名健康对照的CYP1A1 MspⅠ基因型，分析各基因型及与吸烟的交互作用和肺癌易感性的关系。结果：吸烟者的肺癌风险显著增加(0R＝1．87，95％CI：1．10—3．19)(P＜0．05)；MspⅠ突变型在病例组、对照组中各占39．56％、28．26％，其个体的肺癌风险增加(0R＝1．53，95％CI：0．81—2．88)，而杂合型则降低(0R＝0．83，95％CI：0．43--1．60)，携带一个以上突变基因的个体肺癌风险增加(0R＝1．15，95％CI：0．66—2．00)；分层分析发现，吸烟的突变型个体肺癌风险显著增加(0R＝2．56，95％CI：1．05—6．25)(P＜0．05)，而不吸烟对突变型(0R＝0．84，95％CI：0．11—6．21)，尤其是杂合型(0R＝0．27，95％CI：0．09—0．80)(P＜0．05)基因个体有明显的保护作用。结论：CYP1A1 MspⅠ突变型基因是吸烟者肺癌易感性的遗传标记，而杂合型基因可降低非吸烟者的肺癌风险。

Genetic polymorphisms of CYP1A1MspⅠ and susceptibility of lung cancer in Guangdong population

Objective: To explore the relationship between the genetic polymorphisms of CYP1A I MspI and susceptibility of lung cancer in Guangdong Province. Methods Case - control study was conducted, 91 primary lung cancer cases were selected, 91 hospitalize patients and 47 health people are two control groups. The genetic polymorphisms were determinatd with the method of PCR. Results: The frequency of the lung cancer in the smoking individuals was significantly different from those of controls(P<0. 05), (OR 1. 87,95%CI: 1. 10~3. 19);between the lung cancer group and control group,the frequencies of mutant genotypes C were 39. 56% and 28. 26%,the risk of lung cancer was increased (OR=1. 53, 95%CI:0. 81 ~2. 88),but heterozygote B showed a low risk effect(OR = 0. 83,95%CI:0. 43~1. 60),the odds ratio of the lung cancer in the individuals who carrying variant allele was 1. 15(95%CI:0. 66 ~ 2. 00)and shows risk increased;stratified analysis suggested genotype C raised the risk of lung cancer in smoking individuals (OR=2. 56,95%CI;1. 05 ~6.25),(F<0. 05),but in the non -smoking population,genotype C(OR=0. 84,95%CI:0.11~ 6. 21) and heterozygote B(OR=0. 27,95%CI:0. 09-0. 80)(P<0. 05) showed low risk effects. Conclusion: Genotype C is one of the susceptibly genetic markers of lung cancer in the smoking population,but in the non-smoking ones with heterozygote B,the risk of lung cancer can be decreased.