| 胃癌组织线粒体DNA D-loop区的突变及其意义 |
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| 引用本文： | 赵永刚,纪祥瑞,杨堃.胃癌组织线粒体DNA D-loop区的突变及其意义[J].青岛大学医学院学报,2010,46(3):225-228. |
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| 作者姓名： | 赵永刚 纪祥瑞 杨堃 |
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| 作者单位： | 1. 青岛大学医学院附属医院病理科,山东青岛,266003
2. 青岛大学医学院附属医院中心实验室,山东青岛,266003 |
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| 摘 要： | 目的研究胃癌组织中线粒体DNA(mtDNA)D-loop区突变情况及其在肿瘤发生和发展中的作用。方法选择17例胃癌及相应正常胃黏膜组织,应用聚合酶链反应(PCR)对其mtDNA D-loop区进行扩增并测序,将测序结果与线粒体文库中的Revised Cambridge Reference Sequence(rCRS)进行对比分析。结果 17例胃癌组织中共发现mtDNA D-loop区存在175个多态性变异,其中8个(4.6%)为新发现的变异。8例(47.1%)胃癌组织中共发现13次突变;突变热点集中在HV1(23.1%)和HV2(53.9%),并且HV2较HV1更易突变。mtDNA D-loop区突变率与胃癌分化程度、浸润深度、有无淋巴结转移、病人性别和年龄无关(P>0.05)。结论 mtDNA D-loop区尤其是其中的HV1及HV2是一个具有高度多态性和突变性的区域,在胃癌中突变率较高。
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| 关 键 词： | DNA 线粒体 胃肿瘤 突变 多态性 单核苷酸 |
D-loop MITOCHONDRIAL DNA MUTATION IN GASTRIC CANCER AND ITS SIGNIFICANCE |
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| ZHAO YONG-GANG,JI XIANG-RUI,YANG KUN.D-loop MITOCHONDRIAL DNA MUTATION IN GASTRIC CANCER AND ITS SIGNIFICANCE[J].Acta Academiae Medicinae Qingdao Universitatis,2010,46(3):225-228. |
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| Authors: | ZHAO YONG-GANG JI XIANG-RUI YANG KUN |
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| Institution: | ZHAO YONG-GANG,JI XIANG-RUI,YANG KUN (Department of Pathology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China) |
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| Abstract: | Objective To study the mitochondrial DNA(mtDNA) mutation in D-loop region in gastric cancer and its effect on tumorigenesis and development. Methods Polymerase chain reaction(PCR) and sequencing were applied to detect the D-loop region of mitochondrial genome in 17 gastric cancer specimens and corresponding non-cancerous tissue of the stomach.The sequence was compared with the Revised Cambridge Reference Sequence in Mitomap. Results In the tissue of 17 cancer cases,175 polymorphisms were found in D-loop reg... |
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| Keywords: | DNA mitochondrial stomach neoplasms mutation polymorphism mononucleotide |
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