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目的检测慢性阻塞性肺疾病急性加重期（AE—COPD）患者呼吸道内具核梭杆菌（h．）的定植，探讨n．与AE—COPD间的关系。方法选择2008年10月至2009年4月中国医科大学附属第一医院、盛京医院确诊为AE—COPD的患者53例，采集呼吸道分泌物，提取细菌DNA，应用SYBR。GreenIPremixExTaq＇…模式的实时PCR技术，对n．进行定量检测，并计算其所占总菌的比例。同时记录患者FEVl占预计值比例（％）及口腔牙周指标。结果53例样本中有32例检出Fn．，Fn．检出率为60．38％，占总菌的比例为（44．58±16．47）％。Fn．相对含量与FEVl占预计值比例呈负相关关系（P〈0．05）。F凡．相对含量与简化口腔卫生指数及附着丧失水平呈正相关关系（P〈0．05）；与牙龈出血指数、探诊深度、缺失牙数无相关性（P〉0．05）。结论AE—COPD患者呼吸道内存在Fn，Fn相对含量随着肺功能的减弱而增加；Fn．与AE—COPD密切相关。

Detection and study of Fusobacterium nucleatum in the acute exacerbation of chronic obstructive pulmonary disease.

Objective To detect the presence of Fusobacterium nucleatum （Fn.） in the acute exacerbation of chronic ob- structive pulmonary disease（AE-COPD）and to investigate its relationship with FEV1 and periodontal index. Methods The sputum samples of AE-COPD were collected and were extracted DNA of bacterium. The amount of Fn. and the to- tal number of bacterial cells were determined by real-time PCR based on SYBR Green 1 Premix Ex TaqTM. FEV1 and periodontal parameters of each AE-COPI） were recorded, and then investigate the relationship between Fn. and FEV1 and periodontal index. The data were statistically analyzed using SPSS17.0 software. Results The detection rate of Fn. was 60.38% and the relative quantity of Fn. was （44.58＋ 16.47）%. There was negative correlation between the relative quantity of Fn. and FEV1 （P 〈 0.05）. There was positive correlation between the relative quantity of Fn. and simplified oral hygiene index and clinical attachment loss（P 〈 0.05）, Conclusion Fn. can colonize in the sputum samples in the AE-COPD and colonization of Fn. is relevant to FEV1 and periodontal parameters.