当归多糖对小鼠腹腔巨噬细胞释放细胞效应分子的诱导作用

目的 :观察当归多糖对小鼠腹腔巨噬细胞产生细胞毒效应分子的影响。方法 :从BALB/c小鼠的腹腔分离巨噬细胞 ,并进行原代细胞培养。采用MTT比色法及紫外分光光度法 ,检测当归多糖对腹腔巨噬细胞释放一氧化氮 (NO)、肿瘤坏死因子α(TNF α)、活性氧 (ROS)以及诱导型一氧化氮合酶 (iNOS)和溶菌酶 (LSZ)活性的影响。结果 :当归多糖可激活巨噬细胞释放NO、TNF α和ROS等效应分子 ,并显著提高LSZ的活性。当归多糖可能通过提高巨噬细胞iNOS的活性而增加NO的释放量 ,但其与脂多糖 (LPS)无协同促进NO释放的作用。当归多糖体外无直接杀伤肿瘤细胞的作用 ,但其与巨噬细胞共孵育的培养上清具有杀伤L92 9细胞的作用。结论 :当归多糖可促进巨噬细胞释放NO、TNF α及ROS等细胞效应分子 ,并可通过作用于巨噬细胞而促进TNF α的分泌 ,发挥间接的抗肿瘤免疫作用

The role of Angelica polysaccharides in inducing effector molecule release by peritoneal macrophages

AIM: To observe the effect of Angelica polysaccharides on effector molecule production by peritoneal macrophages. METHODS: Macrophages were isolated from the peritoneal cavity of BALB/c mice and the primary culture was performed. MTT colorimetry and spectrophotometry were used to examine the effects of Angelica polysaccharides on the releases of effector molecules, such as nitric oxide(NO), tumor necrosis factor-alpha(TNF-alpha), and reactive oxygen species(ROS) as well as inducible nitric oxide synthase (iNOS) and lysozyme(LSZ) activity by peritoneal macrophages. RESULTS: Angelica polysaccharides could promote the releases of NO, TNF-alpha and ROS from macrophages and improved iNOS and LSZ activities in macrophages. However, Angelica polysaccharides had no direct cytotoxicity to tumor cells, but the cultural supernatant of macrophages cocultured with Angelica polysaccharides could kill L929 cells. CONCLUSION: Angelica polysaccharides can promote the releases of NO, TNF-alpha and ROS by macrophages. Angelica polysaccharides may indirectly play the role of anti-tumors through increased TNF-alpha production by macrophages.