| 脑胶质瘤端粒酶活性与亚单位表达的相关性 |
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| 引用本文： | 张凯,王忠诚,张亚卓. 脑胶质瘤端粒酶活性与亚单位表达的相关性[J]. 中华神经外科杂志, 2000, 16(3): 180-182 |
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| 作者姓名： | 张凯 王忠诚 张亚卓 |
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| 作者单位： | 张凯（100050 北京市神经外科研究所） 王忠诚（100050 北京市神经外科研究所） 张亚卓（100050 北京市神经外科研究所） 何乐（100050 北京市神经外科研究所） 张显峰（白求恩医科大学第一临床学院） |
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| 摘 要： | 目的 检测脑胶质瘤组织中端粒酶阳性及其亚单位的表达，研究端粒酶活性与各亚单位表达的相关性。方法 对６例正常脑组织和５１例胶质瘤组织利用端粒重复序列扩增技术（ＴＲＡＰ）检测了端粒酶的活性，利用ＰＴ－ＰＣＲ法对端粒酶不同亚单位的表达情况进行研究。结果 端粒酶活性检测６例正常脑组织均为阴性，５１例胶质瘤组织中１９例阳性。ｈＴＲ亚单位在６例正常脑组织中５例为阳性，在５１例胶质瘤标本中４８例为阳性，ｈＴＲＴ
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| 关 键 词： | 脑胶质瘤 端粒酶 TRAP RT－PCR 亚单位表达 |
| 修稿时间： | 1999-07-11 |
Correlative study of telomerase activity amd telomerase subunits expression in glioma |
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| ZHANG Kai,WANG Zhogncheng,ZHANG Yazhuo. Correlative study of telomerase activity amd telomerase subunits expression in glioma[J]. Chinese Journal of Neurosurgery, 2000, 16(3): 180-182 |
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| Authors: | ZHANG Kai WANG Zhogncheng ZHANG Yazhuo |
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| Affiliation: | ZHANG Kai,WANG Zhogncheng,ZHANG Yazhuo(Beiuing Neurosurgical Institute. Beijing 100050) |
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| Abstract: | Objective To detect telomerase activity and telomerase subunits expression in glioma samples, to study the correlation of telomerase activity and its subunits expression.Methods In 6 samples of normal brain tissue and 51 samples of glioma tissue, TRAP method was used detect telomerase activity, RTPCR method was used to detect the expression of its three subunits. Results Telomerase activity could not be detected in any of the 6 normal brain tissue samples, while 19 glioma tissue samples showed telomerase activity(373%). In the 6 normal brain tissue samples, 5 cases expressed hTR, none of them expressed hTRT and all cases expressed TP1/TLP1. In the 51 glioma samples, hTR expression were found in 48, hTRT expression in 21 and TP1 expression in 38, Correlation study showed that the subunit hTRT had a strong correlation with telomerase activity in glioma samples(P<0005), whereas hTR and TP1/TLP1 expression showed no correlation with telomerase activity.Conclusion Parts of glioma samples show telomerase activity. hTRT subunit may be the ratelimiting subunit in the activation of telomerase. |
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| Keywords: | Glioma Telomerase TRAP RT-PCR |
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