中药复方干预3周模拟失重大鼠骨丢失的初步研究

目的：研究中药复方对3周模拟失重大鼠骨骼改变的干预作用,初步观察模拟失重情况下,复方对钙剂（牡蛎醋酸水解物）的协同作用。方法：Wistar大鼠,雄性,30只,随机分为3组,空白组、模型组、中药组,每组10只。尾吊模拟失重3周,中药复方（含熟地黄、怀牛膝、黄芪、当归、牡蛎醋酸水解物等）水煎剂灌胃。观察中药对模拟失重大鼠血清钙与磷水平、后身骨密度、骨矿盐含量、力学强度的影响。结果：模拟失重3周后,与空白组比较,模型组血清钙磷均显著升高（P〈0.01）,后身骨密度、骨力学强度均显著降低（P〈0.01）;中药组血钙磷升高程度有减小趋势,骨密度显著提高（P〈0.01或0.05）,骨力学强度有较大改善趋势。结论：3周尾吊模拟失重可造成大鼠后身骨量显著丢失和强度下降,中药可较好地改善上述情况。单纯补充钙剂不足以对抗模拟失重所致骨丢失,复方中熟地黄、怀牛膝、黄芪、当归等可能对钙剂牡蛎起到了协同作用。

Effect of Chinese herb medicine compound on bone loss in rats under 3 weeks simulated weightlessness:preliminary study

Objective: To investigate the effect of Chinese herb medicine compound on bone lose in rats under 3 weeks simulated weightlessness,and to observe the synergistic action of other ingredients in the compound on calcium. Methods: Thirty male Wistar rats were divided into 3 groups:control group,tail-suspend group,tail-suspend and medicine group which took Chinese herb medicine compound(contains Radix Rehmanniae Praeparata,Radix Achyranthis Bidentatae,Radix Astragali,Radix Angelicae Sinensis,Concha Ostreae prepared by acetic acid)by gastric administration. After 3 weeks simulated weightlessness,serum calcium(Ca),phosphorus(P),bone mineral density(BMD) and content(BMC),bone mechanical properties(MEC) were observed. Results: At the end of the experiment,serum Ca and P increased significantly(P<0.01),BMD and BMC of posterior body decreased significantly(P<0.01) in tail suspend rats,compared with in the control group. In rats of tail suspended and medicine group,the increase degree of serum Ca and P were smaller,BMD and BMC of posterior body increased significantly(P<0.01 or 0.05),and MEC also tend to increase. Conclusion: Bone mass of posterior body lose significantly,with mechanical property significantly decrease in rats after 3 weeks simulated weightlessness. Chinese herb medicine compound is effective to prevent the change of bone. Simple calcium supplement can not prevent simulated weightlessness induced bone loss,therefore other ingredients in the compound may perform synergistic action to calcium(Concha Ostreae prepared by acetic acid).