高血压大鼠血管外膜血管紧张素转化酶活性与胶原关系的初步研究

目的通过观察自发性高血压大鼠(SHR)组和Wistar大鼠组血管外膜血管紧张素转化酶(ACE)活性的改变,了解高血压大鼠血管外膜RAS系统激活的情况及与胶原代谢的关系,探讨外膜在血管重塑及功能调控中的可能机制。方法自发性高血压大鼠10只(雄性,20周龄),为实验组。正常Wistar大鼠10只(雄性,20周龄,为对照组。每周采用大鼠血压心率测定仪测定大鼠安静清醒状态下鼠尾动脉收缩压。取胸主动脉行病理形态学观察,其外膜用分光光度法测定血管胶原含量和ACE酶活性。结果发现在高血压大鼠的外膜中的ACE酶活性较正常的Wistar大鼠活性明显升高,两者之间差异有统计学意义,血管外膜ACE酶活性与血管外膜胶原含量有一定的相关性(r=0.65)。结论高血压大鼠主动脉外膜RAS系统激活,ACE酶活性升高,与血管的胶原增生有明显关系,血管外膜RAS在高血压血管重塑中可能起着重要的作用。

Effects of Angiotension I-Converting Enzyme Activity on the Aorta Adventitial Remodeling

Objective To investigate the relationship between adventitia activation and collagen metablosim in rat via the observation of the changes of the adventitial angiotension I-converting enzyme(ACE)activity in the spontancous hypertensive rats(SHR)and Wistar rats,and the possible mechanism of blood vessel remodeling and functional regulation of adventitia.Methods Ten 20 weeks old male spontancous hypertensive rats were used for the experiment group and ten 20 week old male Wistar rat were used for the control group.Blood pressure and heart beat of the rats were measured by tail-cuff method every week.Pathomorphology of the main artery was studied and blood vessel collagen content and ACE activity were determined by spectrophotometer.Results ACE activity of the adventitia of the SHR was significantly higher than that of Wistar rats,and the adventitialy ACE activity was correlated with adventitial collagen level(r=0.65).Conclusion The adventitia of the spontenous hypertensive rat was activated;ACE activity was increased and related to the increase of adventitial collagen.The activation of adventitia in the hypertensive blood vessel remodeling is important.