Studies on LH modulated 8kDa peptide involved regulation of testosterone production in rat Leydig cells |
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Authors: | Ramaraj P Rao A J |
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Affiliation: | Department of Diabetes, Endocrinology & Metabolism, The University of Hope National Medical Centre, Duarte, CA 91007, USA. |
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Abstract: | Aim: To demonstrate the role of the 8 kDa peptide in regulation of testosterone production by mt Leydig cells. Meth-ods: A peptide similar to 8 kDa peptide purified from immature rat Leydig cells was isolated and purified from rat lungcytosol. Immunological and structural similarity between the peptides purified from lung and Leydig cells was estab-lished by Western blot and tryptic map comparison respectively. Results: Addition of the 8 kDa peptide 10, 50, 100;and 150 ug decreased the production of testosterone in Leydig cells dose-dependently. But the addition of the peptide150 ug along with hCG had no effect on hCG-stimulated increase in testosterone production. Conclusion: In vitro ad-dition of the peptide purified from lung cytosol to adult rat Leydig cells resulted in a concentration-dependent decrease inbasal testosterone production although it had no effect on hCG-stimulated testosterone production. (Asian J Androl1999 Dec; 1: 191-194) |
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Keywords: | Leydig cells testosterone regulation differentiation steroidogenesis peptides LH |
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