过氧化氢酶基因-262C/T及-21A/T多态性与燃煤型氟中毒相关性的研究

目的 探讨过氧化氢酶(CAT)基因启动子区-262C/T、-21A/T多态性与燃煤型氟中毒之间的关系.方法 2007年,在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取150名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取150名村民作为干预组,于非地方性氟中毒长顺县白云山镇抽取150名村民作对照组,应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)分析方法,分别检测各组CAT-262C/T、-21A/T多态的基因型.结果 非干预组、干预组和对照组CAT-262C/T、CAT-21A/T多态的基因型频率分布符合Hardy-weinberg遗传平衡定律(P＞0.05).在研究人群中检测到CAT-262C/T多态的两种基因型CC和CT,未检测到TT基因型;检测到CAT-21A/T多态的三种基因型AA、AT和TT.对照组、干预组和非干预组CAT基因-262C/T多态位点的基因型(CC、CT)频率分别为89.33%(134/150)、10.67%(16/150)、88.67%(133/150)、11.33%(17/150)、9333%(140/150)、6.67%(10/150);对照组、干预组和非干预组C等位基因频率分别为94.67%(284/300)、94.33%(283/300)、96.67%(290/300);T等位基因频率分别为5.33%(16/300)、5.67%(17/300)、3.33%(10/300).对照组,干预组和非干预组CAT基因-21A/T多态位点基因型(AA、AT、TT)频率分别为48.67%(73/150)、46.00%(69/150)、5.33%(8/150)、52.67%(79/150)、38.00%(57/150)、9.33%(14/150)、51.33%(77/150)、38.00%(57/150)、10.67%(16/150);对照组、干预组和非干预组A等位基因频率分别为71.67%(215/300)、71.67%(215/300)、70.33%(2ll/300);T等位基因频率分别为28.33%(85/300)、28.33%(85/300)、29.67%(89/300).CAT-262C/T和CAT-21A/T基因型频率及等位基因频率在对照组,干预组和非干预组中的分布,组间比较差异均无统计学意义(x2=0.331、0.336,P均＞0.05).结论 CAT基因-262C/T、-21A/T多态性与燃煤型氟中毒无明显的相关性.

Abstract：

Objective To explore the relationship between -262C/T and -21A/T polymorphisms of catalase(CAT) gene and coal-burning borne fluorosis. Methods In 2007, 150 villagers were taken as a nonintervention group in Bijie city from the village of coal-burning borne fluorosis areas with unchanged cooking stoves;150 villagers were taken as the intervention group from the town of Changchun county where cooking stoves changed; 150 villagers were taken as control from non-endemic fluorosis areas in Baiyun town of Changshun county.PCR-restriction fragment length polymorphism were employed to detect genotypes of CAT-262C/T and CAT-21A/T polymorphism of CAT gene. Results The genotypic frequencies of CAT-262C/T and CAT-21A/T in nonintervention group,intervention group and control group were in line with Hardy-Weinberg equilibrium law (P＞ 0.05 ).The genotypes of CC and CT were detected while no TT were detected for CAT-262C/T polymorphism; the genotypes of AA, AT and TT were detected for CAT-21A/T. The genotype frequencies of CAT-262 CC, CT in control group, intervention group and non-intervention group were (89.33%(134/150), 10.67%(16/150); 88.67%(133/150), 11.33% (17/150),93.33% (140/150),6.67% (10/150), respectively. The gene frequency of C in control group, intervention group and non-intervention group were (94.67% (284/300), 94.33% (283/300),96.67%(290/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 5.33%(16/300), 5.67%(17/300), 3.33%(10/300), respectively. The genotype frequencies of CAT-21 AA,AT and TT in control group, intervention group and non-intervention group were 48.67%(73/150),46.00%(69/150),5.33%(8/150) ,52.67%(79/150) ,38.00%(57/150) ,9.33% (14/150) ,51.33%(77/150) ,38.00%(57/150), 10.67%(16/150), respectively. The gene frequency of A in control group, intervention group and non-intervention group were 71.67%(215/300),71.67%(215/300),70.33%(211/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 28.33% (85/300),28.33% (85/300),29.67% (89/300),respectively. CAT-262C/T and CAT-21A/T genotype and allele frequencies in the control group, the intervention group and non-intervention group showed no significant differences in the distribution(x2= 0.331,0.336, all P ＞0.05 ). Conclusion CAT-262C/T and CAT-21A/T polymorphism is not associated with coal-burning borne fluorosis.

Relationship between-262C/T and-21A/T polymorphism of catalase gene and coal-burning borne fluorosis

Objective To explore the relationship between -262C/T and -21A/T polymorphisms of catalase(CAT) gene and coal-burning borne fluorosis. Methods In 2007, 150 villagers were taken as a nonintervention group in Bijie city from the village of coal-burning borne fluorosis areas with unchanged cooking stoves;150 villagers were taken as the intervention group from the town of Changchun county where cooking stoves changed; 150 villagers were taken as control from non-endemic fluorosis areas in Baiyun town of Changshun county.PCR-restriction fragment length polymorphism were employed to detect genotypes of CAT-262C/T and CAT-21A/T polymorphism of CAT gene. Results The genotypic frequencies of CAT-262C/T and CAT-21A/T in nonintervention group,intervention group and control group were in line with Hardy-Weinberg equilibrium law (P＞ 0.05 ).The genotypes of CC and CT were detected while no TT were detected for CAT-262C/T polymorphism; the genotypes of AA, AT and TT were detected for CAT-21A/T. The genotype frequencies of CAT-262 CC, CT in control group, intervention group and non-intervention group were (89.33%(134/150), 10.67%(16/150); 88.67%(133/150), 11.33% (17/150),93.33% (140/150),6.67% (10/150), respectively. The gene frequency of C in control group, intervention group and non-intervention group were (94.67% (284/300), 94.33% (283/300),96.67%(290/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 5.33%(16/300), 5.67%(17/300), 3.33%(10/300), respectively. The genotype frequencies of CAT-21 AA,AT and TT in control group, intervention group and non-intervention group were 48.67%(73/150),46.00%(69/150),5.33%(8/150) ,52.67%(79/150) ,38.00%(57/150) ,9.33% (14/150) ,51.33%(77/150) ,38.00%(57/150), 10.67%(16/150), respectively. The gene frequency of A in control group, intervention group and non-intervention group were 71.67%(215/300),71.67%(215/300),70.33%(211/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 28.33% (85/300),28.33% (85/300),29.67% (89/300),respectively. CAT-262C/T and CAT-21A/T genotype and allele frequencies in the control group, the intervention group and non-intervention group showed no significant differences in the distribution(x2= 0.331,0.336, all P ＞0.05 ). Conclusion CAT-262C/T and CAT-21A/T polymorphism is not associated with coal-burning borne fluorosis.