An investigation of purified tetanus toxoid by the method of precipitation in agar

Summary Several antigens were detected in the purified tetanus antitoxinby the method of precipitation in the agar. These antigens gave different distribution in the fractions obtained by fractional precipitation of this antitoxin by the phosphate mixture (pH=7.4). Precipitation of specific antigen (antitoxin) was differentiated by comparison of the data obtained by reaction of precipitation in the agar with the data of antitoxin-fixating reaction obtained in experiments on white mice. Relatively greater thermolability of the specific antigen compared with the admixed ballast antigens served as an auxiliary factor in this test.Presented by Active Member Acad. Med. Sci. USSR, G. V. Vygodchikov