月腺大戟水提物对小鼠的毒性作用

目的：探讨月腺大戟水提物的毒性作用。方法:以实验用一级标准昆明种近交系小鼠为实验对象,随机分为5组。阴性对照组，给予蒸馏水灌胃；阳性对照组，腹腔注射环磷酰胺50mg·kg^-1；低、中、高剂量组，分别给予77、144、288g·kg^-1月腺大戟水提物灌胃，观察微核率、精子畸变率以及高剂量组的肝、脾、肾、心、脑、肺病理切片。结果:高剂量(288g·kg^-1) 月腺大戟水提物对脾脏、肾脏和心脏具有毒性作用；中、高剂量组的微核率和精子畸变率与阴性对照组均有统计学差异(P＜0.01, P＜0.005)，低剂量组与阴性对照组无统计学差异(P＞0.05)。结论:月腺大戟水提物在较高剂量下有致突变和生殖毒性，但在低剂量下未发现明显毒性。

Toxicity of Euphorbia ebiacteolata Hayata water extract on mice

Objective: To investigate the potential toxicity of Euphorbia ebiacteolata Hayata (EEH) water extract. Methods: The study was performed on inbred Kunming mice, using the traditional toxicological method based on observing ratios of micronucleus and sperm abnormity (RMS). The mice were randomly divided into 5 groups: a negative control with distilled water, a positive control with cytoxan(50mg·kg^-1), and low dose, medium, and high dose of EEH extract(77g·kg^-1, 144g·kg^-1 and 288g·kg^-1 respectively). Results: The results showed that the high and medium doses significantly increased RMS compared with the negative controls (P＜0.01, P＜0.005), but a similar result was not observed in the low dose group (P＞0.05). Conclusion: EEH can induce mutation and sperm abnormity in medium and high doses, whereas it cannot induce significant toxicity in low doses.