大鼠脑挫裂伤后c-jun和c-fos基因快速反应的特点

背景:目前关于c-jun和c-fos基因表达的重要性已逐渐被人们认识,而关于脑挫伤后两种基因表达的特点报道较少。目的:探讨脑挫裂伤后c-jun和c-fos基因表达的特点。设计:随机对照实验研究。地点和材料:实验地点:原张家口医学院实验中心电镜室,成年Wistar大鼠45只,体质量200～310g。干预:将45只大鼠随机分为模型组、假手术组及正常对照组,正常组不做任何处理,假手术组开颅窗不进行打击,模型组大鼠以自由落体法复制脑挫伤模型,伤后30min,1,2,4,8,24,72h杀死大鼠取脑组织,石蜡切片,用PV6000免疫组织化学染色。主要观察指标:c-jun和c-fos基因蛋白表达的异同点。结果:正常对照组和假手术组c-fos无阳性表达,而c-jun呈弱阳性。模型组c-fos阳性神经元分布在伤灶周围区皮质1.0～2.0mm和同侧海马;c-jun阳性神经元在伤侧皮质和海马广泛分布,相邻脑片c-jun阳性细胞着色深,尤其在海马中c-jun阳性细胞密集而核深,72hc-fos基本上已无表达,而c-jun仍有较明显的阳性神经元。结论:脑挫伤后,同侧脑组织c-jun基因表达较c-fos更强烈、更广泛和表达时间更长。

Quick response of genes c-jun and c-fos after brain injury in rats

BACKGROUND: The importance of gene expressions of c-jun and c-fos has been widely recognized. However, there are very few reports on the characters of the expressions of these two genes after brain contusion.OBJECTIVE: To explore the characters of gene expressions of c-jun and c-fos.DESIGN: Randomized case control study.SETTING and MATERIALS: Experimental location: Experimental Centre of Zhangjiakou Medical College. Forty-five adult Wistar rats, with a boby mass about 200 g to 310 g.INTERVENTIONS: Forty-five rats were randomly divided into model group, sham operation group and normal control group. No treatment to normal group. The skulls were opened without strike for sham operation group. Free falling body method was used to make the brain injury model for model group. Rats were executed in 0. 5, 1, 2, 4, 8, 24 and 72 hours. Brain tissues were taken out to make paraffin sections and dyed by PV6000 immunohistochemistry.MAIN OUTCOME MEASURES: The similarities and differences of gene expressions of c-jun and c-fos.RESULTS: There was no positive expression of c-fos in the normal and sham group. However, there was weakly positive of c-jun. In the model group, the positive neurons distributed in the 1.0 - 2.0 mm cortex area surrounding the focus and the hippocampus of the same side. c-jun positive neurons were widely found in the cortex of injury side and hippocampus. The color of c-jun positive cells was dark, especially in hippocampus which c-jun positive cells concentrated. There was hardly expression of c-fos in 72 hours, but there were obvious positive neurons of c-jun existed.CONCLUSION: The gene expression of c-jun in the same side of brain injury is much stronger and more extensive than that of c-fos. The expressive time is much longer.