大鼠精神分裂症后自发性运动量及海马神经发生的改变

目的:探讨成年大鼠精神分裂症后自发性运动量和海马神经发生的改变。方法:通过连续2周腹腔注射环苯已哌啶(phencyclidine,PCP)建立大鼠精神分裂症模型,利用动物运动分析系统监测大鼠自发性运动量,5-溴-2-脱氧尿苷嘧啶(BrdU)标记新生的神经细胞,用免疫荧光标记法监测海马齿状回BrdU、NeuN、S-100β的表达,利用激光共聚焦显微镜观察海马神经细胞的增殖与分化情况。结果:精神分裂症模型大鼠比对照组大鼠自发性运动量高出2～3倍(P0.05);BrdU阳性细胞数约下降了24%(P0.05);两组BrdU阳性细胞的分化无明显差异性(P0.05),大多分化为神经元。结论:精神分裂症可导致成年大鼠自发性运动量增加,并引起海马神经发生的改变,降低神经细胞的增殖。

Changes of locomotor activities and hippocampal neurogenesis in rats subject to schizophrenia

Objective:To investigate the changes of counts of locomotor activities and hippocampal neurogenesis in adult rats subject to schizophrenic.Methods:Rats received intraperitoneal injection of phencyclidine (PCP) daily for 14 consecutive days for schizophrenic model.Locomotor activities were recorded using a Supermex behavior analyzing system.The newly generated neurocytes were labeled by bromodeoxyuridine (BrdU).The expression of neuronal nuclei (NeuN)、S-100β and the number of BrdU positive cells in hippocampal dentate gyrus were detected by immunofluorescence histochemical staining.Results:The counts of locomotor activities in schizophrenic model rats were increased 2-to 3-fold compared to that of control group(P<0.05) and displayed a significant reduction by 24% in BrdU-positive cells compared with that of control group (P<0.05).No significant difference in the differentiation of BrdU-positive cells were noted (P>0.05)and most of BrdU-positive cells differentiated into neurons in both groups.Conclusion:The schizophrenic could cause counts of locomotor activities increase and lead to changes of hippocampal neurogenesis and inhibit proliferations of neurons of adult rats.