胃癌及癌前病变组织中PCNA、Cyclin D1及P27的表达

目的研究与细胞增殖及周期相关的PCNA、Cyclin D1和P27在胃癌及癌前病变组织中的表达。方法采用组织芯片技术构建包含正常胃黏膜组织、肠化生、异型增生、胃癌组织的组织芯片．组织诊断由病理科医师对每一点进行检查、确定。用免疫组化技术研究PCNA、Cyclin D1和P27从正常胃黏膜到胃癌组织的表达变化趋势。结果PCNA和Cyclin D1在胃癌、异型增生、肠化生组织中表达均较正常胃黏膜增高；P27在胃癌、异型增生、肠化生组织中的表达较正常胃黏膜降低。结论PCNA、Cyclin D1和P27在胃癌及癌前病变组织的表达与正常胃黏膜比较均有较大差异，且有逐渐增加或减少的趋势，均与胃癌的演变密切相关。

Tissue microarray for detecting the expression of PCNA, Cyclin D1 and P27 during the course of gastric carcinogenesis

Objective To detect the expressions of PCNA, Cyclin D1 and P27, which were related to cell proliferation and cell cycle in gastric carcinoma and precancerous lesion of gastric mucosa. Methods Tissue microarray technique was applied to construct a tissue microarray containing gastric carcinoma, precancerous lesions and normal gastric mucosa. Diagnosis of the tissue in the microarray was pathologically confirmed. Immunohistochemical method was used to assess the expression of PCNA, Cyclin D1 and P27 in all the samples of tissue microarray. Results The expressions of PCNA and Cyclin D1 in gastric carcinoma, gastric hyperplasia, and intestinal metaplasia tissues were higher than that in normal gastric mucosa. The expressions of P27 in gastric hyperplasia, intestinal metaplasia and gastric carcinoma tissues were lower than that in normal gastric mucosa. Conclusion Gastric carcinoma, precancerous lesion and normal gastric mucosa tissues present different degrees of expression of PCNA, Cyclin D1 and P27, either with a increasing trend or a decreasing trend. These changes in expression are all closely related to gastric carcinogenesis.