川芎嗪对BLM所致大鼠肺纤维化保护作用的电镜研究

目的 从细胞学超微结构变化观察川芎嗪对肺间质纤维化的治疗作用.方法采用气管内注入BLM方法制做肺间质纤维化动物模型.留取肺组织制备电镜标本.结果电镜可见：7天BLM组大鼠Ⅰ型肺泡上皮细胞受损,上皮基底膜水肿、裸露甚至断裂;Ⅱ型肺泡上皮细胞增生,微绒毛破坏,稀少或消失,其内板层小体增多增大,空泡样变,且分泌到肺泡腔内,线粒体肿胀,嵴断裂或消失甚至出现空泡样变.肺间质及肺泡腔内炎性细胞增多,可见巨噬细胞和淋巴细胞浸润,内含大量溶酶体及吞噬颗粒,7天即可见肺间质内束状胶原纤维.14、28天BLM组Ⅱ型细胞较7天BLM组减少,间质纤维组织增生明显,见大量纵横交错的胶原纤维束和纤维母细胞增生.7天川芎嗪组炎性细胞浸润较7天BLM组减轻,肺泡Ⅱ型细胞少数板层小体空泡样变,微绒毛存在且较丰富,而14、28天川芎嗪组肺间质中胶原原纤维总量不多.结论本文从超微结构变化证实了川芎嗪减轻BLM所致氧自由基对大鼠肺组织的损伤及纤维化程度.

The Protective Effect of Ligustrazine on Blomycin-induced Pulmonary Fibrosis

Objective It has been well known that oxygen free radical play an important role in causing pulmonary fibrosis.The purpose of this study is to evaluate the protective effect of ligustrazine on blomycin-induced pulmonary fibrosis.Methods By observing pathological changes of lung tissues,the effect of ligustrazine was in investigated on the pulmonary fibrosis rats.Results Electron microscopic findings showed that:In day 7 groups,alveolar type I cell were degenerated broken-down and desqamated.Endothelial cell swelled and alveolar type II cells became hyperplastic,basement membrane thinned and deformed,mast cell were infiltrated and degranulated in day 14 and 28 groups,myofibroblasts,fibroblasts,collage and basement membrane likematerial were haperplasia.Conclusions The above results show that ligustrzine play an important role in treating pulmonary fibrosis due to its antioxidation.But is has no effect on formed fibrosis.