Blood group genotyping in multi-transfused patients |
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| Authors: | Sule Mine Bakanay Aysenur Ozturk Talia Ileri Elif Ince Suzan Yavasoglu Nejat Akar Zumrut Uysal Onder Arslan |
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| Institution: | 1. Yale University School of Medicine, New Haven, CT;2. VA Connecticut Healthcare System, West Haven, CT;3. New York Blood Center, New York, NY;4. Emory University School of Medicine, Atlanta, GA;1. Immunology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt;2. Hematology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt;3. Clinical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt |
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| Abstract: | BackgroundIn chronically transfused patients, the classical hemagglutination assays may be inaccurate in defining the RBC phenotypes of the patients due to previous transfusions.DesignDNA samples from 39 multi-transfused patients including thalassemia and sickle cell disease were used for red blood cell genotyping. The Rh-Type and KKD-Type (BAGene, BAG Healthcare) were used to determine the polymorphisms associated with antigen expression for RHD, RHCE and Kell, Kidd, Duffy blood group systems, respectively. Results were compared with previously determined phenotyping results for RhD, RhCcEe and Kell by hemagglutination method.ResultsNineteen out of the 37(51%) patients had discrepancies between genotyping and phenotyping results in a total of 25 alleles. In 12 patients, the discrepancies had the potential of alloimmunization.ConclusionBlood group genotyping has vital importance in transfusion management of chronically transfused patients especially if the patients were not phenotyped before starting the initial transfusions. |
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