The ex vivo effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on rat intra- and extraneuronal monoamine oxidase activity |
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| Authors: | Y Arai Y Toyoshima H Kinemuchi T Tadano K Kisara |
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| Affiliation: | 1. School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, NSW, Australia;2. Melbourne Water Corporation, Docklands, VIC, Australia;3. School of Civil and Environmental Engineering, Faculty of Engineering, University of New South Wales, Sydney, NSW, Australia;1. College of Safety Science and Engineering, Nanjing Tech University, Nanjing, Jiangsu 210009, China;2. Research Centre for Fire Safety Engineering, Department of Building Services Engineering, The Hong Kong Polytechnic University, Kowloon, Hong Kong;1. Postgraduate Program in Animal Science, Laboratory of Nutrition and Fish Feeding (AQUANUT), State University of Santa Cruz (UESC), 45662-900 Ilhéus, BA, Brazil;2. Federal Institute of Education, Science and Technology Baiano (IFBaiano), Campus Uruçuca, 45680-000 Uruçuca, BA, Brazil;3. State University of Southwest Bahia (UESB), Campus Jequie, 45206-190 Jequie, BA, Brazil;4. Federal University of the Valley of Sao Francisco, (UNIVASF), Petrolina, PE, Brazil;1. Natural Resources Institute Finland (Luke), Tietotie 4, FI-31600 Jokioinen, Finland;2. Department of Environmental Systems Science, Swiss Federal Institute of Technology, ETH-Zurich, Tannenstrasse 1, 8092 Zurich, Switzerland;3. Department of Agricultural Sciences, University of Helsinki, Koetilantie 5, P.O.Box 28, FI-00014, University of Helsinki, Finland |
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| Abstract: | After i.p. injection of 30 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) daily for 4 days and sacrificing the rats 4 h after the last injection, striatal monoamine oxidase (MAO)-A and -B activities, assayed by conventional method with 5-hydroxytryptamine (5-HT) and benzylamine, were not changed. By an uptake technique, with dopamine as the substrate for both uptake and MAO, intrasynaptosomal MAO-A and -B activities were found to be greatly reduced with a greater MAO-A reduction. Intrasynaptosomal 5-HT oxidation by MAO-A was not changed in other forebrain regions treated with these MPTP doses. Similar results were also found with two brain preparations treated with single MPTP doses (30 mg/kg). This reduction in intrasynaptosomal MAO activity was completely absent after treatment with lower MPTP doses (15 mg/kg, daily for 5 days) and 5 days of a withdrawal period. The decrease in MAO activity might have been due to the decrease in DA transport into striatal synaptosomes during the enzyme assay and/or to reversible inhibition of intrasynaptosomal MAO by MPP+. |
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