|
|||||
|
|
Differentiation between steroid hormone receptors CBG and shbg in human target organ extracts by a single-step assay |
|
| Authors: | RK Wagner PW Jungblut |
| Institution: | Max-Planck-Institut fur Zellbiologie, D-2940 Wilhelmshaven, Postfach 1009 G.F.R. |
| Abstract: | The steroid complexes of (plasma) corticosteroid-binding globulin can be distinguished from intracellular steroid-receptor complexes by agar electrophoresis at low temperature in neuraminidase-treated tissue extracts. With this method, the presence of progesterone receptor has been demonstrated in heavily plasma-protein-contaminated human uterus “cytosol”, but not in human mammary carcinoma extracts. SHBG and “basic” receptors for estradiol and dihydrotestosterone in human uterus cytosol could also be assayed simultaneously. |
| Keywords: | steroid-binding plasma proteins cytoplasmic receptors neuraminidase agar electrophoresis Estradiol (E-2) l 3 5(10)-estratriene-3 17β-diol dihydrotestosterone (DHT) 17β-hydroxy-5α-androstan-3-one progesterone 4-pregnene-3 20-dione cortisol 11β 17α 21-trihydroxy4-pregnene-3 20-dione D-norgestrel 13-ethyl-17α-ethinyl-17β-hydroxy-4-gonen-3-one PPO 2 5-diphenyloxazol POPOP 1 4-tris(5-phenyl-2-oxazolyl)benzene neuraminidase CBG corticosteroid-binding globulin SHBG sex-hormone-binding globulin |
| 本文献已被 ScienceDirect 等数据库收录！ | |